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- 人胚胎腎細胞HEK293HEK293
- 將pGL3-OCP-shLuc與pGL3-control分別共轉染人胚胎腎細胞HEK293和人骨肉瘤細胞MG-63,48h后測定蟲熒光素酶活性,計算相對活性。After confirmed by dou ble restriction enzyme digestion and DNA sequencing,the resulting plasmid pGL3 OCP shLuc was used to co transfect HEK293 and MG 63 cells with pGL3 control. Luciferase activities were assayed 48 hours after co transfection,and the relat ive activities were calculated.
- 利用重組質粒pAAV/EGFP與包裝/幫助質粒pDG共轉染人胚腎細胞HEK293,生產重組的AAV病毒。HEK293 cells were transfected with pAAV/EGFP and PDG containing all AAV and Ad functions required for amplification and packaging of AAV vector plasmids.
- 人胚胎腎細胞human embryonic kidney cell
- (3)將CTGF基因克隆進入腺病毒質粒載體中形成重組腺病毒質粒Adeasy-CTGF,并將重組腺病毒質粒Adeasy-CTGF導入包裝細胞HEK293中形成表達CTGF的高滴度重組腺病毒Ad5-CTGF。Results: (1) Picked out the same targe gene CTGF of Wnt and BMP signaling pathways using cDNA microarray, (2) Cloned the CTGF gene in vitro and verified the true result by gene screening, (3) Intergrated the CTGF into the plasmid vecter of adenovirus and result the recombinant ade-plasmid Adeasy-CTGF, (4) Transfected the Adeasy-CTGF into the package cell HEK293 and generated the high titer adenovirus expressing the CTGF.
- 組織塊法體外培養人胚胎神經干細胞Tissue Piece Culture of Human Embryonic Neural Stem Cells in Vitro
- 人胚胎肝細胞株human-derived fetal hepatoeytes
- 人胚胎干細胞建系及培養的研究進展Research progress on human embryonic stem cell lines
- 體外沖擊波碎石術對腎細胞內鈣水平和腎細胞凋亡的影響Effects of extracorporeal shock wave lithotripsy on calcium ion level and apoptosis in renal cells
- 人胚胎多能干細胞研究中的幾個問題Several problems in studying human embryonic pluripotent stem cells
- 腎細胞癌在透析病人的表現有別于一般病人而有獨特的表現。Because of the loss of renal excretion of excess calcium, hypercalcemia may be the first observed sign in dialysis patients with RCC.
- 一種簡便高效的人胚胎干細胞轉染方法A Simple and Efficient Transfection Way for Human Embryonic Stem Cell
- 猴腎細胞MK cells; monkey-kidney cells
- 人胚胎干細胞的體外原代培養條件分析Study of condition for culturing human primary embryonic stem cells
- 胚腎細胞HEK-293
- 人胚胎神經干細胞的分離培養及鑒定研究Isolation, cultivation and characterization of neural stem cell from human fetal cerebral cortex
- 田鼠腎細胞body hamster kidney cell
- 人胚胎皮層神經前體細胞的分離培養及鑒定Isolation and identification of neural precursor cells from human embryonic cortical tissue
- 成腎細胞瘤Wilm tumor
- 人胚胎期表皮干細胞與汗腺發生過程關系的研究Study on interrelation between epidermal stem cells and developing process of sweat gland cells in human fetal skin