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- It is concordant with routine PCR method. 并與常規PCR法具有很好的一致性。
- The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method. 因此,我們利用接頭PCR技術(shù)克隆了PNZIP基因的啟動(dòng)子。
- The applications of Mullis' PCR method are already many. 原理簡(jiǎn)介:PCR技術(shù)的基本原理是DNA的半保留復制。
- A multiplex- PCR method for detection bovine materials in foodstuffs. 動(dòng)物飼料中牛成分的復合PCR檢測方法。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR檢測細胞周期素D1(cyclinD1)、細胞周期蛋白依賴(lài)激酶 4 (CDK4 )mR NA的表達。
- Meanwhile, the PCR method is superior to immunohistochemistry in detecting HPV. 在檢測上,PCR方法優(yōu)于免疫組織化學(xué)染色,值得提倡和推廣。
- Using PCR method, BBTV or CMV can be detected from the banana leaf tissue equivalent to 100 ng. 用PCR方法可從相當于100ng的葉片組織中檢測到BBTV和CMV。
- Objective To develop a PCR method for the determination of porcine parvovirus(PPV). 目的建立豬細小病毒(PPV)PCR檢測方法。
- The results prove that adapter ligation PCR method is workable to clone carnation CHS promoter. 然后利用銜接頭PCR的方法進(jìn)行了香石竹CHS啟動(dòng)子克隆的初步研究,得到了約500bp和800bp的PCR產(chǎn)物,目前克隆測序工作正在進(jìn)行。
- This is to develop a rapid,sensitive and specific fluorescence PCR method detecting listeria monocytogenes in food. 發(fā)展一種快速、敏感、特異的熒光PCR方法檢測食品中單核細胞增生李斯特氏菌。
- In order to improve diagmostic procedure of E. suis, the PCR method was established. 鑒于上述原因,為了建立特異準確的診斷方法,本研究開(kāi)展了附紅細胞體病的聚合酶鏈式反應(PCR)診斷方法的研究。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了靈敏、特異的檢測家犬糞便中細粒棘球絳蟲(chóng)的PCR方法。
- PCR method was considered as the gold standard, which showed the highest specificity (100%) and sensitivity (100%). PCR法是檢測MRSA的金標準,特異性和靈敏度均為100%25。
- The NDI gene in mtDNA was amplified with regular PCR method, and the PCR product was sequenced after purified. 用PCR擴增人線(xiàn)粒體NDI基因片段,PCR產(chǎn)物經(jīng)純化后測序和核苷酸同源性分析。
- Conclusions PCR method in which 139-141 as primer is suitable for rapid detection of Salmonella. 結論在沙門(mén)菌的快速檢測中,宜采用以139-141為引物的PCR檢測法。
- Objective:This study was to establish a immuno in situ PCR method for the diagnosis of HCMV active infection. 目的:建立用于診斷HCMV活動(dòng)性感染的原位免疫PCR方法。
- Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate. 結論 建立了檢測惡性瘧原蟲(chóng)的熒光定量PCR方法,較常規PCR技術(shù)更為簡(jiǎn)便、快速、準確,有很好的應用前景和研究?jì)r(jià)值。
- According to the sequence of GAPDH gene available in GenBank,a SYBR Green I dye based on real-time PCR method is developed. 根據GenBank中鴨GAPDH基因的序列設計引物;建立了基于SYBR Green I染料技術(shù)的real-time PCR方法.
- All of the 51 Bt were analyzed by the PCR method with general primers of cry1, cry2, cry3, cry4, cry-n, cry7, cry8, cry11, cry13 and cyt genes. 對51 株Bt 分離菌株的cry 基因進(jìn)行了PCR 鑒定。
- Six truncated Cryllel proteins were induced in Ecoli BL21(DE3) containing six deleted fragments from cryllel gene by PCR method. 本研究通過(guò)PCR擴增的方法,以cry1Ie1全長(cháng)基因為模板,分別得到不同末端缺失的基因片段,轉化大腸桿菌BL21(DE3)中,誘導表達,得到6種末端缺失的蛋白。