two- step freezing的用法和樣例：
例句
- As far as cryostorage of recalcitrant seeds is concerned, vitrification and two-step freezing have been applied in many studies.
頑拗性種子的低溫保存，應用較多的是玻璃化法和兩步法。 - Alkyl polyglucosides were prepared from glucose and lauryl alcohol by one step reaction using p-toluenesulfonic acid and dodecyl benzene sulfonic acid as catalysts respectively.
分別采用對甲苯磺酸和十二烷基苯磺酸為催化劑進(jìn)行了一步法由葡萄糖和月桂醇制備烷基糖苷的工藝研究。
