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- The Tat signal peptide detecting vector of PGA were constructed. 構建了Tat信號肽檢測載體。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考慮到克隆方便,在序列5’端和3’端分別引入BamHI和EcoRI 位點(diǎn),并在兩個(gè)酶切位點(diǎn)的外側分別加上保護堿基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信號肽是疏水的，與膜上結合的蛋白接近，拉著(zhù)后面的親水多肽跨膜，一旦跨過(guò)膜，即被信號肽酶切除，釋放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 應用神經(jīng)網(wǎng)絡(luò )算法和隱馬氏模型分析結果顯示該肽鏈信號肽位置為1-26，斷裂點(diǎn)位置是26-27（GAA-AF）。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外區由758個(gè)氨基酸組成，包括N端31個(gè)氨基酸的信號肽和七個(gè)類(lèi)似免疫球蛋白(Ig)的結構域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推導的貓IL-18氨基酸序列中,無(wú)信號肽序列和潛在的N-聯(lián)糖基化位點(diǎn),但存在4個(gè)Cys殘基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在質(zhì)粒pET-20b(+)中，重組的蛋白會(huì )在N-末端帶有一個(gè)信號肽序列，它可以使重組蛋白分泌到細胞的周質(zhì)空間處，而其在C-末端還有帶有6個(gè)組氨酸的標簽，這樣就使得重組蛋白的分離純化更加的方便，簡(jiǎn)單。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周質(zhì)中，或通過(guò)Bayer接點(diǎn)分泌到胞外環(huán)境中的蛋白有一個(gè)信號肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通過(guò)在CEMA的N端添加信號肽，對其進(jìn)行改造，以探索不降低抑菌作用的同時(shí)，降低其對植物細胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2編碼區分別長(cháng)821bp和859bp，由于編碼區內存在提前終止密碼子和插入/缺失引起的移碼突變，推測其為不表達的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通過(guò)TMHMM2.;0(http://genome
- Fibroblast growth factor（FGF-1）lacks amino-terminal signal peptide, so it can’t release through the classical endoplasmic reticulum（ER）-Golgi pathway. 酸性成纖維生長(cháng)因子-1（FGF-1）由于氨基端缺乏信號肽序列，不能通過(guò)經(jīng)典的內質(zhì)網(wǎng)-高爾基體途徑釋放。
- The mother with twin babies are always on the run. 這個(gè)有雙胞胎嬰兒的媽媽總是忙忙碌碌的。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常規的分子生物學(xué)技術(shù),從小鼠骨髓細胞中克隆了含信號肽序列的SCF,并構建了表達載體。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文將從信號肽結構特征、常用的信號肽序列以及構建高分泌型載體等方面進(jìn)行綜述。
- The twin brothers are far apart in character. 那兩個(gè)孿生兄弟的性格相差很遠。
- Signal peptide was found on former 20 amino acids of both two coding regions of protein, one strong and one feeble transmembrane helices were also found. 2. 并在兩種酶蛋白編碼區的前20個(gè)氨基酸發(fā)現信號肽序列，通過(guò)跨膜區預測，均發(fā)現一個(gè)較強的跨膜信號和一個(gè)較弱的跨膜信號。
- You must be muddling me up with my twin brother. 你一定是把我看成我的孿生兄弟了。
- The fusion DNA fragment of the lactococcal extracellular protease USP45 signal peptide, passenger protein and SBC were expressed in Lactococcus lactis and Lactobacillus paracasei. 融合乳酸菌胞外蛋白酶USP45訊息勝肽、乘載蛋白及表層蛋白質(zhì)靜默基因C端123個(gè)胺基酸，表現于副乾酪乳桿菌或乳酸鏈球菌。
- Nothing can set the twin brothers at variance. 沒(méi)有什么事能使這對雙胞胎兄弟吵起來(lái)。
