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- 融合Kan~r基因fused kanamicin resistance gene
- 經(jīng)卡那霉素(Kan)的初步篩選,獲得了29株抗Kan的再生植株。After the preliminary selection of Kan, we get 29 anti-Kan regenerated plantlets.
- 通過(guò)離體葉片再生的Kan梯度篩選實(shí)驗，將轉化芽的選擇選壓力定為40mg/l Kan。The survival pressure of Kan for the regeneration of transgenic shoot was confirmed as 40mg/l by the gradient antibiotic resistance experiment.
- 利用農桿菌介導法,將植物表達載體pBLGT35Svp4-st和pBLGvp4-st轉化苜蓿,經(jīng)卡那霉素(Kan)多重篩選,獲得抗性植株。The promoter of T35S was linearized with pBLGvp4-st. Plant expresses vector pBLGT35svp4-st was constructed by enzyming, linearizing and translating. Alfalfa contained the plant expression vector pBLGT35svp4-st and pBLGvp4- st by Agrobacterium-mediated transformation and antibiotics( Kan) many filtrate.
- 目的:比較康樂(lè )霉素C(Kan)和環(huán)孢素(Cyc)對十四酰佛波醇乙酯(TPA)和伊屋諾霉素(IM),及刀豆球蛋白A(Con A)誘導淋巴細胞增殖的作用。AIM: To compare the suppressions of kanglemycin C (Kan) with that of cyclosporine (Cyc) on lymphocyte proliferations induced by tetradecanoylphorbol acetate (TPA) with ionomycin (IM), and concanavalin A (Con A).
- 然后依次轉入附加Kan 50mg/L+Cef 500mg/L的MSB_1、MSB_2、MSB_3培養基中進(jìn)行篩選，獲得抗性植株。 兩個(gè)品種的莖尖轉化率均為7.89%±。Subsequently, the explants were placed in turn onto MSBi, MSBz , MSBs containing Kan 50mg/L and Cef 500mg/L to be screened, and the transformants were obtained with transformation frequency amounting to 7.89%25.
- 在以前的Xcc致病比較與功能基因組學(xué)研究工作中,通過(guò)EZ::TN
Tnp轉座子誘變Xcc 8004獲得一個(gè)插入位點(diǎn)在XC3814 CDS (protein coding sequence)內的突變體。 In our previous functional genomic study, Xcc 8004 was randomly inserted with a transposon EZ::TNTnp. A pathogenicity-deficient mutant with insertional site in the CDS (protein coding sequence) XC3814 was identified. - 采用正交旋轉設計方法對影響芥藍遺傳轉化體系的因素進(jìn)行了優(yōu)化研究,結果表明:影響芥藍KanR苗率的最主要因素是Kan濃度,而預培養時(shí)間和共培養時(shí)間是芥藍遺傳轉化的主要影響因素。By the orthogonalty design,the factors which influenced the transformation of Brassica oleracea L.var. alboglabra have been studied,the results showed that the major factor for KanR frequency is Kan concentration,and the major factor for Chinese kale transformation are pre-culture time and co-culture time.
- 在建立麝香百合葉片再生體系的基礎上,研究了Mn-SOD基因轉化中影響葉片轉化效率的多種因素,包括Kan濃度、抑菌素濃度、浸染菌液等,從而確立了麝香百合‘white elegance’的遺傳轉化體系。On the base of regeneration system from leaf explant of "white elegance", several factors that affected genetic transformation of the Easter Lily cultivars were examined, including selection of Kan concentrations, the suspension of strain EHA105, concentrations of Carb, and so on. Thus a simple and efficient genetic transformation system was developed.
- 轉化煙草的同時(shí)，利用農桿菌介導的浸漬法轉化擬南芥(Columbia ecotype)，得到T_0代種子，將種子干燥后，于4℃放置兩周，再于加有50mg/l的Kan的1/2MS上篩選陽(yáng)性植株，目前這一步她在進(jìn)行當中。Arabidopsis (Columbia ecotype) was transformed by floral dip method mediated by Agrobacterial and TO seeds have been harvested. The antibiotic screening of positive Arabidopsis plant is carrying out now.
