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- Special RNA molecules stop the synthesis of proteins from a target gene (top); other genes are unaffected (bottom). 特殊的RNA分子正在制止靶基因合成蛋白質(zhì)(頂);其他的一飲不受影響(底)
- Analyse the genotype of the target genes using PCR. 聚合酶鏈反應(PCR)擴增目的基因進(jìn)行基因型分析。
- The silencing of target gene transcription by unliganded NRs(including AR) may be mediated by these corepressors. 非配體化的核受體(NRs)抑制靶基因的轉錄可能是通過(guò)這些輔阻遏子來(lái)介導的。
- Epitope based vaccine design is adopted and chemical method is used to synthesis target genes. 方法采用基于表位的疫苗設計方法，用化學(xué)合成的方法制備該種分子。
- Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. 應用RT-PCR或RQ-PCR方法在石蠟包埋組織中檢測目的基因的表達是可行的。
- The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction. 將mdlA基因克隆到原核表達載體pET-28a中，轉化大腸桿菌BL21(DE3)，經(jīng)誘導后，重組的脂肪酶在宿主菌中的表達量可達菌體總蛋白量的45.;2%25。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 結論:成功構建了帶有目的基因的原核表達載體。
- The therapy of antiangiogenesis and target gene offer a broad clinic prospect for lung cancer. 內皮抑素的抗血管生成治療和血管靶向基因治療,為治療肺癌提供了廣闊前景。
- Target gene combined with proper vector is transformed in Ecoli cells to amplify. 用適當的載體與目的分子連接后，轉入大腸肝菌宿主細胞擴增。
- A previous study has mapped the Ihd gene onto chromosome 10 with SSR markers, but the linked markers were found on one side of the target gene. 先前的研究利用SSR標記已將lhd基因定位在第10染色體上，但只在目標基因一側找到標記。
- RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用減蛋綜合征陽(yáng)性血清在體外中和重組腺病毒，接種HEK293細胞；
- METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. 方法 :從正常前列腺組織中釣取PSP94cDNA ,構建重組質(zhì)粒pUC19-PSP94 ,轉化后提取目的基因并進(jìn)行測序。
- The sequence of target gene of recombinant plasmid pcDNA3-ALR constructed in this experiment was in accordance with the sequence of rat ALR cDNA reported. 構建的pcDNA3-ALR重組質(zhì)粒目的基因與文獻報道的大鼠ALR cDNA序列相同。
- With RNA (ribonucleic acid) interference mechanism, scientists have found a new type of anti-AIDS drugs may contribute to the large number of R &D target gene. 借助RNA(核糖核酸)干擾機制，科學(xué)家最近找到了可能有助于新型抗艾滋病藥物研發(fā)的大量目標基因。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前認為，高血壓是多種遺傳因素和環(huán)境因素共同作用而引起的復雜性狀疾病，在人類(lèi)遺傳易感性的基礎上，在環(huán)境危險因素的作用下發(fā)病。
- Gene therapy is the hot spot currently.This article reviews the progression of anti-neovascularization of retina and chorioid in aspect of animal model,vector and target gene. 基因治療是目前研究的熱點(diǎn),我們從動(dòng)物模型、載體、治療性靶基因的選擇等方面對視網(wǎng)膜和脈絡(luò )膜新生血管治療的進(jìn)展進(jìn)行綜述。
- A novel shuttle plasmid which can be replicated in yeast and expressed in mammal cell is required in oral gene therapy with yeast as vector to deliver target gene. 為研究酵母作為載體在口服基因治療及免疫中的作用,需要一種能夠在酵母中復制而在哺乳動(dòng)物細胞中表達的穿梭載體。
- RNA interference (RNAi) is the process of sequence-specific gene silencing, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the target gene. 由乙型肝炎病毒（Hepatitis B virus, HBV）感染引起的慢性乙型肝炎是嚴重危害人類(lèi)健康的疾病，其主要危害在于HBV感染造成的慢性持續性感染易引起肝硬化，并與肝細胞肝癌（Hepatocellular carcinoma， HCC）的發(fā)生密切相關(guān)。
- A conditional expression model, in which the target gene is deleted in the presence of a tetracycline derivative, was used to delete the VEGF gene from podocytes. 一個(gè)有條件的表達模型用來(lái)從足突細胞中刪除VEGF基因，在四環(huán)素衍生物的出現下靶基因被刪除。
- Liver Stem Cells as Vector for Targeted Gene Therapy of Hepatocellular Carcinoma. 肝干細胞作為肝細胞癌靶向基因治療載體的實(shí)驗研究
