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- It turns that most of the recombinant protein were inclusion body. 對重組蛋白的細胞內定位分析發(fā)現蛋白幾乎全部以包涵體形式存在。
- RESULTS: Most of the product didn t form inclusion body in cell. 結果:表達產(chǎn)物在細胞內大部分沒(méi)有形成包含體。
- The chicken inclusion body hepatitis virus could induce SPF chicken to synthesize TNF with the infection by mouth. 研究結果表明,雞包涵體肝炎病毒經(jīng)口腔感染SPF雛雞可刺激機體產(chǎn)生TNF,在感染的不同日齡雞的TNF活性出現一定的消長(cháng)規律。
- Objective To investigate the clinical and pathological features of inclusion body myositis (IBM). 摘要目的探討包涵體肌炎的臨床與病理特點(diǎn)。
- Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法：對包涵體進(jìn)行有效洗滌，采用疏水層析進(jìn)行純化。
- The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body. 對含人EC-SOD重組質(zhì)粒的工程菌進(jìn)行誘導表達形成包涵體。
- Objective:To investigate the clinical and pathological features of inclusion body myositis(IBM). 目的探討包涵體肌炎(IBM)的臨床表現與病理特點(diǎn)。
- The results showed that there was a silver-stainedDNA area(inclusion body,IB)in every cell infected with MCV. 結果顯示：在被感染的皮膚表皮細胞內都有一個(gè)大小及構型不同的銀染區（病毒工廠(chǎng)）。
- Objective To choose the metal ions of immobilized metal affinity chromatography for separating the inclusion body of recombinant hepatitic E virus. 目的選擇固相金屬親和層析柱的金屬離子,以?xún)?yōu)化分離純化重組戊型肝炎病毒包涵體的條件。
- Several immune system cell types and processes have been recently identified in muscle in inclusion body myositis, dermatomyositis, and polymyositis. 近來(lái)，包涵體肌炎、皮肌炎、多發(fā)性肌炎中幾種免疫細胞類(lèi)型和免疫反應過(guò)程被認定。
- The application of Hofmeister effect in recombinant product inclusion body denaturation and renaturation in biotechnology is discussed in particular. 目前重組蛋白生產(chǎn)過(guò)程中包含體的變性和復性問(wèn)題為上述理論研究提供了廣泛的應用空間。
- The virus replicated in the intracytoplasm and formed inclusion bodies. 病毒在細胞漿內復制，可形成特征性包涵體；
- In the multiplicating process, Lysosome constructs inclusion body of baculovirus by engulfing mitochondrias and pathogens, using the energy supplied by mitochondrias. 溶酶體在增殖過(guò)程中內吞瀕臨解體的線(xiàn)粒體和病原體，利用線(xiàn)粒體內嵴提供的能量，有規律地組裝成桿狀病毒的包涵體。
- Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分離包涵體，確定1 M尿素洗滌包涵體為分離MP-GFP的最優(yōu)條件。經(jīng)Ni-NTA樹(shù)脂純化目標蛋白，SDS-PAGE電泳分析其純度達到90%25以上。
- The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%). VH和VL重組質(zhì)粒分別轉化到大腸肝菌BL21（DE3）中，IPTG誘導后，經(jīng)SDS?PAGE電泳表明在12kD處有包含體蛋白表達，表達蛋白含量分別為28%25和35%25。
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表達產(chǎn)物為包涵體形式，溶解和折疊后，以GST親合層析色譜純化。
- With anti-CDV antigen assay, the ependymal cells with inclusion bodies showed an intensive positive reaction. 部分錐體細胞，神經(jīng)核細胞和浦氏細胞變性、溶解或胞漿深染，胞核濃縮。
- Swimming is the best way to tone up your body. 游泳是健體強身的最好方法。
- It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. 它也包括關(guān)于包涵體，熔化蛋白質(zhì)和結晶學(xué)的洗凈的洗凈的章。
- The monolayer BEF with CPE was stained by HE, and the inclusion bodies were found in the enlarged cell nuclei. CPE表現為細胞腫大、變圓、脫落，HE染色可見(jiàn)到病變細胞內有核內包涵體。
