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- The anti-mutagenicity of reduced coenzyme NADH was studied by Ames assay and single cell gel electrophoresis assay (SCGE, comet assay). 采用Ames實(shí)驗及單細胞凝膠電泳(SCGE,彗星實(shí)驗),對還原型輔酶NADH進(jìn)行抗突變研究。
- The single cell gel electrophoresis(SCGE) also called commet assay is a method which can detect DNA rupture under level of single cell. 單細胞凝膠電泳(SCGE)又稱(chēng)為彗星實(shí)驗,是一種可以在單個(gè)細胞水平上檢測DNA斷裂的技術(shù)。
- Objective: Traditional Ames test, in vitro mammalian chromosome aberration test, single cell gel electrophoresis assay have been used widely in monitoring chemical toxicity. 目的:傳統的Ames實(shí)驗、體外哺乳動(dòng)物細胞染色體畸變實(shí)驗、單細胞凝膠電泳實(shí)驗已廣泛用于化學(xué)品毒性的監測,但用于綜合評價(jià)化學(xué)品的遺傳學(xué)毒性還不夠完善。
- The single cell gel electrophoresis(SCGE),also called comet assay,is a sensitive and rapid method for DNA damage detection in individual mammalian cell. 單細胞凝膠電泳是一種在單細胞水平檢測哺乳類(lèi)有核細胞DNA斷裂的新技術(shù)。
- The tail extent, tail inertia, percentage of tail DNA and Olive tail moment are selected as parameters, which could be measured by the fluorescent pictures of single cell gel electrophoresis (SCGE) and IMI 1.0 comet assay analysis software. 觀(guān)察指標為彗星狀熒光圖像的彗星尾長(cháng)、尾%25DNA、尾慣量、Olive尾矩。
- Methods DNA damage was tested in HepG2 cells exposed to organic extracts from CDW by using single cell gel electrophoresis assay (SCGE). 方法 氯化飲水有機提取物染毒 Hep G2后 ,用單細胞凝膠電泳 (亦稱(chēng)彗星試驗 )檢測 DNA損傷。
- The comet assay (the alkaline single cell gel electrophoresis) was applied universally in the field of ecotoxicology as a sensitive bioassay to detect DNA damage in the cell level. 彗星試驗，又稱(chēng)單細胞凝膠電泳，因其能夠在單細胞水平上靈敏地檢驗DNA鏈斷裂，成為研究遺傳毒性效應的新技術(shù)，近年來(lái)得到重要應用。
- Single cell gel electrophoresis(SCGE) or comet assay(CA) is a rapid,simple,sensitive,reliable and inexpensive method for detecting DNA damage in eukaryotic individual cells. 單細胞凝膠電泳(SCGE)是一種快速、簡(jiǎn)便、靈敏、可靠的在單細胞水平上檢測真核細胞DNA損傷與修復的技術(shù)。
- single cell gel electrophoresis assay 單細胞凝膠電泳
- single cell gel electrophoresis assay(SCGE) 單細胞凝膠電泳試驗
- Single cell gel electrophoresis(SCGE), which has founded in 1980s, can detect the DNA injury in single cell. 單細胞凝膠電泳技術(shù)建立于20世紀80年代,用于檢測單個(gè)細胞的DNA損傷。
- The ability of trichloroethylene(TCE) to induce single strand breaks in peripheral lymphocytes DNA of male mice in vivo was evaluated using single cell gel electrophoresis(SCGE). 為研究三氯乙烯（TCE）的遺傳毒性，應用單細胞凝膠電泳法（SCGE）測定了TCE誘導人和小鼠外周血有核細胞DNA鏈斷裂的能力。
- Purpose and Methods: Single cell gel electrophoresis(SCGE), sister chromatid exchanges(SCE), chromosome aberrations are three methods to determine DNA damage and repair. 目的與方法 :單細胞凝膠電泳技術(shù) (SCGE)、姐妹染色單體交換法 (SCE)和染色體畸變分析法均能被用來(lái)檢測DNA的損傷或修復。
- Single cell gel electrophoresis is applied for genetic toxicology,DNA damage and detection,environmental pollution detection,apoptosis mechanism research et al. 單細胞凝膠電泳被廣泛應用于遺傳毒理學(xué)、DNA損傷與修復的檢測、環(huán)境污染檢測、細胞凋亡機制的研究等諸多領(lǐng)域。
- The sertoli/germ cell culture and single cell gel electrophoresis (SCGE) was used to detect the DNA damage of spermary cell in mice, which was induced by tested m-nitrobenzene. 采用小鼠睪丸支持細胞/生殖細胞共培養法以及單細胞凝膠電泳技術(shù),研究了間二硝基苯對小鼠睪丸生殖細胞DNA的損傷作用。
- Methods Semen samples from soldiers with different job categories were analyzed by single cell gel electrophoresis(SCGE)and computer assisted sperm analysis(CASA). 方法采用單細胞凝膠電泳和精子全自動(dòng)分析相結合的方法對兩組不同職業(yè)男性的精液標本進(jìn)行檢測。
- Single cell gel electrophoresis is applied for genetic toxicology, DNA damage and detection, environmental pollution detection, apoptosis mechanism research et at. 單細胞凝膠電泳被廣泛應用于遺傳毒理學(xué)、DNA損傷與修復的檢測、環(huán)境污染檢測、細胞凋亡機制的研究等諸多領(lǐng)域。
- DNA damage induced by radon in bone marrow of mice was detected by m ethods o f single cell gel electrophoresis (SCGE), micronucleus(MN) and laser scanning co nfocal microscope (LSCM) observation. 采用單細胞凝膠電泳（SCGE）、微核（MN）實(shí)驗、激光共聚 焦顯微鏡（LSCM）檢測小鼠骨髓細胞的DNA鏈斷裂、微核細胞率及凋亡率，觀(guān)察氡暴露致小 鼠骨髓細胞的DNA損傷。
- Single cell gel electrophoresis(SCGE) is a technique to detect DNA damage of single cell quickly,and is generally applied for reproductive cells. 單細胞凝膠電泳技術(shù)(SCGE)是一種快速檢測單個(gè)細胞DNA損傷的實(shí)驗技術(shù),在生殖細胞DNA損傷的檢測中廣泛應用。
