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- Donors and recipients HLA class I typing was performed using complement dependent cytotoxicity (CDC) test with special monoclonal tray (SMT) and HLA class II gene typing by micro sequence specific primers polymerase chain reaction (Micro PCR SSP). 采用補體依賴(lài)性細胞毒試驗 (CDC)和微量序列特異性引物聚合酶鏈反應 (Micro PCR SSP)技術(shù)進(jìn)行HLA I類(lèi)和II類(lèi)分型。
- Sequence specific primer ( PCR - SSP) 順序特異性引物
- The sequence specific primers of the gene mutation of the mannose combining agglutinator gene exon I at 52 site condon was detected with polymerase chain reaction. 進(jìn)行甘露糖結合凝集素外顯子I52位密碼子基因突變的序列特異性引物聚合酶鏈式反應檢測。
- Polymerase chain reaction- sequence specific primer 聚合酶鏈反應-序列特異引物法
- Sequence specific primer based polymerase chain reaction, PCR-SSP 運用序列特異性引物聚合酶鏈反應
- Polymerase chain reaction with sequence specific primer, PCR-SSP 應用聚合酶鏈反應-序列特異性引物方法
- A~(1,2)BO~(1,2) genotyping by PCR sequence specific primer PCR-SSP法檢測A~(1,2)BO~(1,2)血型基因型
- polymerase chain reacation sequence specific primer (PCR-SSP) 聚合酶鏈反應-序列特異性引物（PCR-SSP）
- Methods HLA?DQA?1 and DQB?1 gene polymorphism was tested in 30 CLT patients and 24 normal controls of Chinese Han nationality by using DNA amplification with polymerase chain reaction of sequence?specific primers (PCR?SSP). 方法用序列特異性引物的聚合酶鏈反應（PCR?SSP）方法，分析30例由病理證實(shí)的我國漢族CLT患者及24名正常對照的外周血白細胞基因組DNA的HLA?DQA1及DQB1位點(diǎn)的基因多態(tài)性分布。
- Evaluating the polygene polymorphism by sequence specific primers polymerase chain reaction in large samples 用序列特異性引物多聚酶鏈反應的方法進(jìn)行大樣本、多基因的多態(tài)性鑒定
- Keywords HLA-DRB1;polymerase chain reaction;sequence specific primers;genotyping;Lahu Chinese; 關(guān)鍵詞HLA-DRB1;聚合酶鏈反應;序列特;異引物基因分型;拉祜族;
- The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. 采用PCR-序列特異性引物技術(shù)(SSP)對HLA-DQB作中分辨法分型。
- Keywords sequence specific primer;polymerase chain reaction;genotyping polymorphism; 序列特異性引物;多聚酶鏈反應;基因多態(tài)性;
- sequence specific primers 序列特異性引物
- Two pairs of specific primers: Tch old and Tch new primers were designed based on the result of 18S rRNA gene sequence. 經(jīng)試驗證明這兩對引物能夠非常有效的區分呂氏泰勒蟲(chóng)和尤氏泰勒蟲(chóng)。
- sequence specific primer 序列特異的引物
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培養酵母TY-1和野生酵母W1,W2的基因組DNA,進(jìn)行Delta-PCR,可以分別得到穩定而獨特的DNA指紋圖譜。
- A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence. 根據苯酚羥化酶基因高度保守序列設計一對該基因的特異引物。
- The ORF1 sequence of gene mrp encoding muramidase-released protein(MRP) was amplified from genomic DNA of Streptococcus suis type 2 Qinghai strain by PCR with specific primers. 根據豬鏈球菌2型溶菌酶釋放蛋白基因(mrp)的序列,設計并合成了1對特異性引物,以青海株的基因組DNA為模板擴增了mrp基因ORF1序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根據已報道的大腸桿菌不耐熱腸毒素（LT）的基因序列設計引物，用PCR方法從Escherichia coli 216株基因組中擴增出LT基因的全序列。
