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- Human immunoglobulin combinatorial library was generated by using phage surface display expression system, and phage antibodies (Fab fragments ) to hepatitis B surface antigen (HBsAg )were screened from it. 應用噬菌體表面遞呈表達系統構建了人抗體組合文庫,并篩選出了結合乙肝表面抗原(HBsAg)的人噬菌體抗體(Fab片段)。
- The ScFv gene was expressed as fusion protein with phage g3p coat protein and displayed on the phage surface. 結果通過(guò)免疫熒光鑒定,這種抗體仍保留著(zhù)親代抗體的特性,能與登革3型病毒發(fā)生特異性結合。
- M13 bacteriophage has been extensively applied in phage surface display technique. M13噬菌體已被廣泛地應用于噬菌體展示技術(shù).
- The techniqu e of GEAs such as ribosome display and phage surface display and their applicati on progress were summarized in this review. 本文將介紹核糖體展示、噬菌體表面展示等基因工程抗體技術(shù)及應用進(jìn)展。
- The phage display antibody library is developed by reasonably combining the antibody library technology and phage surface display system. 將抗體庫技術(shù)與噬菌體表面展示系統有機地結合就發(fā)展成為噬菌體展示抗體庫。
- AIM: To construct a human recombinant immunoglobulin library containing D-Dimer by using phage surface display technology. 目的:應用噬菌體展示技術(shù)構建人源性抗D二聚體噬菌體抗體抗體組合文庫。
- Objective To determine the surface expression of Fas Ag on RBL 2H3 and its function. 目的 研究大鼠肥大細胞表面Fas的表達情況及其功能。
- Utilizing the technology of phage surface display, special antibody can be gained from the human naive Fab phage display library, which can be used as a new therapy for tumors. 本研究構建的人天然Fab段噬菌體抗體庫可為進(jìn)一步篩選特異性抗體,用于免疫治療打下基礎。
- To determine the surface expression of Fas Ag on rat mast cells and its function. 明確大鼠肥大細胞表面Fas表達及其功能;
- The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology. 噬菌體抗體庫技術(shù)是抗體基因文庫技術(shù)和噬菌體表面展示技術(shù)相結合形成的一項新技術(shù)與方法,在生物科學(xué)領(lǐng)域極具潛力。
- Furthermore, by examining CD71 surface expression, we are able to distinguish and differentially isolate anergic and actiated T cells in io. 另外;通過(guò)檢測CD71的胞膜表達;我們能夠在體內鑒別并分離無(wú)能與活化的T細胞.
- Alternatiely, the induction of anergy results in markedly less S6 kinase 1 Thr(421)/Ser(424) phosphorylation and CD71 surface expression. 換之;無(wú)能可導致明顯地S6激酶1在421位蘇氨酸/424位絲氨酸磷酸化和CD71胞膜表達的減少.
- The field is located on a large, gentle WNW-plunging anticline with surface expression, that is cut by numerous NE-trending normal faults. 兩個(gè)狹窄的地塹將儲層分為三個(gè)氣藏,主要的一個(gè)氣藏位于海濱和濱外;
- Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application. 結論制備的可溶性抗Fas?Fab抗體具有抗Fas抗體的特異性,為進(jìn)一步的研究奠定了基礎。
- Background: Platelets stimulate coagulation via surface expression of phosphatidylserine (PS), providing a negatively charged surface for thrombin generation. 背景:凝血通過(guò)刺激血小板表面表達的磷脂酰絲氨酸( PS部門(mén)) ,提供了一個(gè)負電荷的表面凝血酶生成。
- The results indicated that the enchancement of HLA-I heavy chain synthesis and TAPi gene mRNA expression in HBV transfected cells might mediate the up-regulation of HLA-I surface expression. 提示HBV誘導轉染細胞內HLA一A基因重鏈合成量及1人P:基因轉錄水平增高,可上調細胞的HLA一I表面表達。
- Methods: Changes of platelet surface expression of CD41/CD42a, CD36/TSP, CD62/CD63, CD9 and CD31 were measured before and after FLP treatment by using flow cytometer in 30 cases of mid late stage lung cancer. 方法 :采用流式細胞技術(shù)檢測 30例中晚期肺癌患者服肺瘤平二號前后血小板表面糖蛋白CD4 1/CD4 2a、CD36 /TSP、CD6 2 /CD6 3、CD9、CD31表達的改變 ,并與 50名健康志愿者的檢測結果進(jìn)行對照。
- Transfection of ovarian carcinoma cells with the eukaryotic vector pcDNA3 Fas enhanced cell surface expression of Fas gene and induced susceptibility to Fas antibody mediated apoptosis. 經(jīng) pcDNA3 Fas真核表達載體轉染的卵巢癌細胞Fas基因表達及對Fas單抗誘導凋亡的敏感性均有不同程度提高。
- According to work by Geoffrey Collins of Wheaton College and Jason Goodman of the Woods Hole Oceanographic Institution, this basin could be the surface expression of a subsurface sea. 根據美國惠頓學(xué)院的柯林斯(GeoffreyCollins)和伍茲赫爾海洋研究所的古德曼(JasonGoodman)的研究結果,這個(gè)盆地可能是地下海洋存在的徵兆。
- bacteriophage surface expression system 噬菌體表面表達系統