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- Antigen was appraised by peptide mass fingerprint (PMF) matched with Mascot human protein database. PMF was obtained by immunoprecipitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). 用免疫沉淀和基質(zhì)輔助激光解吸電離飛行時(shí)間質(zhì)譜(matrix-assistedlaserdesorption/ionizationtime-of-flightmassspectrometry,MALDI-TOF-MS)得到相應的肽質(zhì)量指紋譜(peptidemassfingerprint,PMF),再通過(guò)Mascot在人類(lèi)蛋白質(zhì)數據庫中分析比對鑒定mAb對應的抗原。
- Selecting differentially expressed proteins to march enzymolysis in gel, detect peptide mass fingerprint by MALDT-TOF-MS, then research data base of Swiss-port to identify the proteins. 選取差異蛋白質(zhì)點(diǎn)進(jìn)行膠內酶解,以MALDI-TOF-MS測肽質(zhì)量指紋圖,檢索Swiss-Port數據庫,對蛋白質(zhì)進(jìn)行鑒定。
- The facility also provides computer termini for peptide mass fingerprinting (PMF) and/or MS/MS ions searching against database. 同時(shí)也提供電腦及資料庫供使用者做資料搜尋之用。
- MALDI-TOFMS has been established as powerful tools for the identification of proteins by peptide mass fingerprinting in proteomics. 由于其信號檢測靈敏、速度快以及種類(lèi)多樣化的優(yōu)點(diǎn),生物質(zhì)譜已經(jīng)成為生物分析領(lǐng)域中最前沿研究如蛋白組學(xué)研究的支撐技術(shù)。
- Keywords Two-dimensional electrophoresis;Mass spectrometry;Peptide mass fingerprint;Epididymis;Proteome; 雙向凝膠電泳;質(zhì)譜;肽質(zhì)量指紋譜;附睪;蛋白質(zhì)組;
- Application of Peptide Mass Fingerprint in Ricin Identification with Matrix-assisted Laser Desorption Ionization Time-of-Flight-Mass Spectrometry 一種基于肽質(zhì)量指紋譜技術(shù)鑒定蓖麻毒素的新方法
- Keywords Ricin;peptide mass fingerprint;matrix-assisted laser desorption ionization time-of-flight-mass spectrometry;biomass spectrometry; 蓖麻毒素;肽質(zhì)量指紋譜;基質(zhì)輔助激光解吸電離飛行時(shí)間質(zhì)譜;生物質(zhì)譜;
- Keywords hybridoma;peptide mass fingerprinting;chimeric antibody; 雜交瘤;質(zhì)譜分析;嵌合抗體;
- Peptide mass fingerprint 肽質(zhì)量指紋譜
- peptide mass fingerprint search 肽質(zhì)量指紋譜搜索
- Keywords Somanase;Peptide Mass Fingerprinting;Squid-type DFPase;Active site; 人肝梭曼水解酶;肽指紋圖譜信息;魷魚(yú)型的DFP酶;活性位點(diǎn);
- Keywords apple juice concentrate;protein;peptide mass fingerprinting;amino acid analysis; 濃縮蘋(píng)果汁;蛋白質(zhì);肽質(zhì)量指紋譜;氨基酸分析;
- Keywords esophageal cancer;proteomics;mass spectrometry;peptide mass fingerprinting;RT-PCR; 食管癌;蛋白質(zhì)組;質(zhì)譜分析;肽質(zhì)量指紋圖;RT-PCR;
- Keywords Biological sample;analysis of protein drug;endostatin;bio-mass spectrometry;peptide mass fingerprinting (PMF);tandem mass spectrometry; 生物樣品;蛋白質(zhì)藥物分析;內皮抑制素;生物質(zhì)譜;肽質(zhì)量指紋譜;串聯(lián)質(zhì)譜;
- Keywords myasthenia gravis;muscle skeletal;P25 protein;immunolocalization;Peptide Mass Fingerprinting;carbonic anhydrase III; 重癥肌無(wú)力;骨骼肌;P25蛋白;免疫定位;肽指紋圖譜;碳酸酐酶III;
- Keywords Parathyroid Hormone;Osteoblast;Two-dimensional Gel Electrophoresis(2-DE);Proteome;Peptide Mass Fingerprinting (PMF); 關(guān)鍵詞甲狀旁腺激素;成骨細胞;雙向凝膠電泳;蛋白質(zhì)組;肽質(zhì)量指紋圖譜;
- Keywords proteome;two-dimensional gel electrophoresis;keratinocyte;Fibroblast;matrix-assisted laser desorption/ionization mass spectrometer;Peptide Mass Fingerprinting; 蛋白質(zhì)組;雙向凝膠電泳;角質(zhì)形成細胞;成纖維細胞;基質(zhì)輔助激光解吸電離飛行時(shí)間質(zhì)譜;肽質(zhì)量指紋圖譜;
- Peptide mass fingerprinting 肽質(zhì)量指紋譜技術(shù)
- A cloud is a mass of vapor in the sky. 云是天空中的水汽團。
- MALDI-TOF MS is used to identify proteins by matching a list of experimental peptide masses with the calculated list of all peptide masses of each entry in a database. 例如,未知蛋白質(zhì)經(jīng)蛋白質(zhì)酵素裂解后,藉質(zhì)譜儀可得到裂解產(chǎn)生之各種勝肽的分子量。它們可和資料庫中已知蛋白質(zhì)預測之勝肽的分子量比對,比對之結果可用以辨識該未知蛋白質(zhì)。