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- Oridonin is the the main antitumor constituents of Donglingcao. 冬凌草甲素是中草藥冬凌草抗腫瘤的主要活性成分。
- Abstract：ObjectiveTo determine the optimal extracting process of oridonin. 目的確定冬凌草中冬凌草甲素的最佳提取工藝。
- Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 測得冬凌草葉中冬凌草甲素含量為0.;6152%25，平均回收率為97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 結果測得冬凌草葉中冬凌草甲素含量為0.;6152%25，平均回收率為97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%,and the average recovery was 97.86% with RSD of 2.14%. 結果測得冬凌草葉中冬凌草甲素含量為0.;6152%25;平均回收率為97
- Abstract: OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 文章摘要: 目的 從冬凌草中提取冬凌草甲素并測定其含量。
- After recrystallizing the oridonin content is determined by TIC Scanning to be as high as 99.28%. 經(jīng)重結晶后用薄層掃描法測定含量達99.;28%25;是一種簡(jiǎn)便易行的方法。
- The oridonin contents can be controlled by control the amout of the leaves in Blushred Rabdosia. 建議通過(guò)控制藥材的含葉量來(lái)控制冬凌草片中冬凌草甲素的含量。
- Methods: It was studied by orthogonal experimental design and oridonin in extraction was determined by HPLC. 方法：通過(guò)正交試驗，以HPLC法測定提取物中冬凌草甲素的含量為指標進(jìn)行研究。
- OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 摘要目的冬凌草中提取冬凌草甲素并測定其含量。
- After recrystallizing the oridonin content is determined by TLC Scanning to be as high as 99.28%. 經(jīng)重結晶后用薄層掃描法測定含量達99.;28%25;是一種簡(jiǎn)便易行的方法。
- METHODS Microwave technology was used to extract oridonin and the content of oridonin was determined by HPLC. 方法采用微波萃取技術(shù)提取,HPLC法測定。
- In the process of large-scale extracting research of oridonin, a large amount of components of pigment and fat were also extracted as impurities. 在進(jìn)行冬凌草規?；崛⊙芯恐?提取冬凌草甲素的同時(shí)還會(huì )夾雜大量色素及油脂類(lèi)雜質(zhì),嚴重干擾冬凌草甲素的分離純化。
- Results:Oridonin could decrease the activity of telomerase, as well as cause apoptosis and inhibit the growth of K562 cells significantly. 并呈現出明顯的量-效與時(shí)-效關(guān)系。
- We assume that the structural modification of oridonin would produce novel drug candidates with improved solubility and higher potency than oridonin. 本文試從藥理作用、構效關(guān)系及其衍生物等方面對冬凌草甲素的研究概況作一綜述。
- Objective: To investigate the change of Bcl-2 expression and telomerase during the apoptosis induced by oridonin on human hepatocelluar carcinoma BEL7402 cells. 目的:探討冬凌草甲素誘導人肝癌細胞BEL-7402凋亡中Bc l-2表達及端粒酶活性的變化。
- Oridonin is an ent-kaurene diterpenoid compound mainly isolated from the leaves of R.rubescen.Oridonin has been proved to possess good antitumor activity. 冬凌草甲素是從冬凌草等植物葉子中提取出的一種貝殼杉烯二萜類(lèi)化合物,具有好的抗腫瘤活性。
- By means of liqid scintillation count technique the effect of oridonin on the incorporation of [~3H] TdR, [~3H] UR and [~3H] leucine into the ECA cells in vitro was studied. 采用[~3H]TdR、[~3H]UR和[~3H]leucine在體外參入ECA細胞的液體閃爍計數術(shù),探討冬凌草甲素對DNA、RNA和蛋白質(zhì)合成的影響。
- Methods:The variation of both morphology and inhibitory rate of K562 cells were observed in culture medium with various concentrations of Oridonin at different time in vitro. 方法:以不同濃度的冬凌草甲素作用于體外培養的K562細胞,檢測細胞生長(cháng)抑制率,觀(guān)察細胞凋亡時(shí)的形態(tài)學(xué)變化,對細胞凋亡前后的端粒酶活性進(jìn)行檢測。
- The separation and prepartion methods of oridonin by medium pressure colunm chromatography have the advantages of fast speed,high efficiency,harmlessness,safety and economy. 采用中壓柱層析法提取分離冬凌草甲素,具有洗脫速度快,分離效率高,溶劑無(wú)毒無(wú)污染,安全、價(jià)廉、易得等優(yōu)點(diǎn)。
