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- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功構建Arresten基因重組質(zhì)粒pBV220-Arr;并可在E.
- The result showed the target protein was successful expressed as a SLH-Ap36 fusion protein. 結果表明,目標蛋白以融合蛋白的形式成功表達。
- After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag. 純化后的的目的蛋白進(jìn)一步復性,BMP-2經(jīng)變性液溶解,裝入透析袋內。
- The reconstructed vector NY-ESO-1-pET-28 could express target protein stably in the soluble fraction of bacterial extract. 構建的原核表達載體NY-ESO-1-pET28a(+)可穩定地可溶性表達NY-ESO-1蛋白;
- The 14-3-3 proteins interact with targeting proteins or two different domains in one targeting protein by phosphoserine /phosphothreonine motifs. 7種不同亞型的高度保守的14-3-3蛋白與人類(lèi)細胞中多種不同的信號通路有密切的關(guān)系。
- Some phages showing low, medium, high specificity to each target protein, lysozyme, insulin and RNase A, had been selected respectively for sequencing. 利用新建立的交替洗脫法,使用其它目標蛋白質(zhì)對噬菌體展示肽庫進(jìn)行篩選,又獲得了對胰島素和核糖核酸酶A具有高親和力的噬菌體。
- By expression vector pHHSM002, tpsl gene also expressed efficiently in E. coli BL21(DE3)PlysS ,the target protein amount to 17.8% in cell. 以pHHSM002為表達載體,tps1基因在大腸桿菌BL21中也得到了高效表達,表達的目的蛋白占菌體總蛋白的17.;8%25。
- Pharmacophore hypothesis(PH) is fundamental to de novo design of peptide ligand based on the structure of target protein. 在基于靶蛋白結構的多肽分子設計中,藥效假說(shuō)是從頭設計方法的基本前提。
- Other uses of microarrays include the screening of large numbers of molecules for their capacity to bind a particular target protein. 其它微點(diǎn)陣的用途包括從大量分子中篩選出能結合特定靶蛋白的分子。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶對SUMO融合表達系統表達的重組蛋白進(jìn)行切割時(shí)沒(méi)有多余氨基酸殘留,因此成為蛋白切割工具的熱點(diǎn)。
- Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分離包涵體,確定1 M尿素洗滌包涵體為分離MP-GFP的最優(yōu)條件。經(jīng)Ni-NTA樹(shù)脂純化目標蛋白,SDS-PAGE電泳分析其純度達到90%25以上。
- A new nuclear power station is on the stocks here. 這座新的核電站在建造之中。
- This old man is a famous nuclear physicist. 這位老人是一位著(zhù)名的核物理學(xué)家。
- Plans for nuclear plants have been declassified. 建造核裝備的計劃已不再保密。
- They decided to establish a nuclear power station. 他們決定修建一所核電站。
- SDS-PAGE results showed that there was a clear target protein band in Mut+ recombinant supernatant after 48 hours of culturing, while a faint band only in Muts recombinant after 72 hours. 結果表明,誘導培養48小時(shí)后,Mut~+重組菌株表達產(chǎn)物在SDS-PAGE膠上顯現出清晰的目的蛋白帶,而Mut~s重組菌株培養72小時(shí)才能顯示微弱的目的帶;
- Nuclear energy remains a double-headed creature. 核能依然是一種有利也有弊的能原。
- Nuclear physics is a closed book to me. 核物理學(xué)是我完全不懂的一門(mén)學(xué)科。
- Nuclear physics is a closed book to most of us. 我們大多數人對原子核物理學(xué)一竅不通。