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- Keywords condyloma acuminata;human papillomavirus;peripheral blood mononuclear cell;late gene;major coat protein;baculovirus expression system;enzyme-linked immunosorbent assay; 尖銳濕疣;人乳頭瘤病毒;外周血單一核細胞;晚期基因;主要衣殼蛋白;昆蟲(chóng)-桿狀病毒表達系統;酶聯(lián)免疫吸附法;
- CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 云南省煙草花葉病毒和黃瓜花葉病毒外殼蛋白基因克隆及序列分析。
- The phylogenic tree of PLRV isolates was established according to the amino acid sequence of coat protein. 依據PLRV外殼蛋白氨基酸序列建立了PLRV不同分離物的系統進(jìn)化樹(shù)。
- The ScFv gene was expressed as fusion protein with phage g3p coat protein and displayed on the phage surface. 結果通過(guò)免疫熒光鑒定，這種抗體仍保留著(zhù)親代抗體的特性，能與登革3型病毒發(fā)生特異性結合。
- This gene codes for the coat protein of a bacteria-infecting virus, or bacteriophage. 這是一種感染細菌的病毒（噬菌體）表面蛋白的基因。
- The inhibition of plant activator protein on RNA and coat protein of tobacco mosaic virus(TMV) was studied. 研究了植物激活蛋白對煙草花葉病毒RNA及外殼蛋白的抑制作用。
- Detection of IgM Antibodies to Coxsackie B Viruses in Viral Myocarditis by Using a Synthetic Pep- tide Antigen of CVB Coat Protein VP1. 應用VP1蛋白的合成肽抗原檢測心肌炎患者血清中柯薩奇B病毒IgM抗體
- Although the virus coat protein was present in the endosperms of the seeds of infected wisteria plants by ELASA tests, it is not seed-borne. 雖然在發(fā)病紫藤種子的胚乳中能檢測到其外殼蛋白的存在，但此分離物卻不能通過(guò)種子傳播。
- It appeared isometric with a diameter of 25 nm,had its coat protein with MW 24 700 dalton,contained three double strand RNA species that is 3.8 kb,1.4 kb,1.1 kb respectively. 病毒呈二十面體 ,直徑約 2 5nm ,有空心和實(shí)心 2種粒子 ,具有分子量為 2 4 7kd的外殼蛋白和長(cháng)度分別為 3 8kb、1 4kb、1 1kb的 3個(gè)雙鏈RNA組分。
- However, other scientists are skeptical and believe that the prion protein is a viral coat protein that protects a small nucleic acid that has not yet been detected. 然而,另外的科學(xué)家表示懷疑，他們相信普利昂蛋白是病毒中用于保護至今還未被檢測到小核酸的衣殼蛋白。
- But it need long period and is apt to be limited by season.Based on coat protein (CP) of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. 血清學(xué)技術(shù)是利用病毒外殼蛋白的抗原性，據特異性的抗體抗原免疫學(xué)反應檢測病毒存在，但制備杭血清需時(shí)間長(cháng)，對含量少和無(wú)蛋白外殼的病毒無(wú)法檢測；
- A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. 以分離自馬鈴薯主栽品種“紫花白”的馬鈴薯卷葉病毒(PLRV)分離物的RNA為模板,用人工合成的引物,用反轉錄和隨后PCR擴增的方法合成了PLRV外殼蛋白(CP)基因的cDNA,并克隆于pUC19中。
- Transgenic tobacco (Nicotiana tabacum L. cv.Xanthi) plants which express coat protein (CP)gene (cp) of potato virus X (PVX) were generated via Agrobacterium tumefaciens-mediated gene transfer. 利用PCR方法獲得了馬鈴薯X病毒(PVX)外殼蛋白(CP)基因(cp);并將其構建到植物表達載體中;利用農桿菌介導的葉盤(pán)法轉化煙草(Nicotiana tabacum L.;)
- The coat protein gene of GPV, a Chinese serotype isolate of BYDV, was used to construct three plant expression vectors, pPPI 2, pPPI 3 and pPPI 5, which contain Act promoter or Emu promoter respectively. 以我國特有的大麥黃矮病毒 GPV株系的外殼蛋白 ( CP)基因為材料 ,設計合成了分別含有 Act啟動(dòng)子或 Emu啟動(dòng)子的植物表達載體 p PPI2、p PPI3和 p PPI5。
- The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively. 設計3對表達引物分別擴增侵染了百合無(wú)癥病毒(lily symptomless virus,LSV),百合斑駁病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外殼蛋白基因,并在大腸桿菌BL21(DE3)plysS中原核表達。
- The skirmish grew into a major battle. 小沖突擴大形成大戰斗。
- Peanut stripe virus-Hongan isolate was obtained from the infected peanut seedling in Hongan County,Hubei Province. Its coat protein gene was amplified by RT-PCR and cloned into pGEM-T Easy vector. 從湖北省紅安縣花生種傳苗中得到花生條紋病毒(Peanut stripe virus,PStV)分離物(PStV-Hongan),提取感病葉片的總RNA,RT-PCR擴增了其外殼蛋白基因(cp),克隆至pGEM-T Easy載體,轉化大腸桿菌并鑒定。
- Some slight incident may touch off a major war. 某個(gè)小事件可能會(huì )引發(fā)大戰爭。
- Surprisingly, some isolates accumulate to high levels but still can’t be detected by ELISA.Sequence analysis revealed distinctive amino acids changed in the coat protein ORF of these isolates. 本論文首先探討酵素連結免疫抗體法準確度的限制因子，發(fā)現病毒含量過(guò)低或是鞘蛋白胺基酸序列有變異，皆為造成ELISA偵測準確度不高之原因。
- Local fighting might spark off a major war. 局部戰斗有可能引發(fā)一場(chǎng)大規模戰爭。
