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- We can get nonmalignant hybrid cells. 我們可以獲得非惡性的雜種細胞。
- Pure red cell aplasia with thymoma: evidence of T cell clone disorder. 胸腺瘤致單純紅細胞再生障礙機制的初步探討。
- A hybrid tulip; a hybrid cell. 雜交郁金香; 雜交細胞
- Interspecies cloning is a new technology with the development of Somatic cell clone technology. 異種克隆技術(shù)是在體細胞克隆技術(shù)成功后發(fā)展的一項新技術(shù)。
- After the plasmid EGFP-C1 was transfected into INPC, the stable cell clone designated INPC/EGFP was isolated by G418 selection. The positive rate of EGFP was observed. 質(zhì)粒EGFP-C1轉染INPC后;經(jīng)G418篩選;挑選細胞克隆;命名為INPC/EGFP;觀(guān)察其EGFP陽(yáng)性率.
- The hybrid cells lines were demonstrted as hybridoma by karyotyping. 經(jīng)染色體核型分析證實(shí)為雜交瘤細胞。
- This review introduce the present situation in the technique progress, the basic theoretic research and the applications of the technology of somatic cell cloning. 本文對哺乳動(dòng)物體細胞克隆技術(shù)研究現狀、理論基礎研究、應用等方面作一綜述。
- A hybrid tulip;a hybrid cell. 雜交郁金香;雜交細胞
- Methods T cell clones were isolated by limiting dilution. 方法通過(guò)有限稀釋法建立T細胞克隆。
- It may survive and give rise to two mononucleate hybrid cells called synkaryons. 可存活并產(chǎn)生兩個(gè)稱(chēng)為合核體的單核的雜種細胞。
- Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen. 目的探討在建立了分泌抗鼠疫F1抗原的單克隆抗體雜交瘤株后,對其進(jìn)行克隆化的實(shí)驗方法。
- This may require isolating a very large number of hybrid cells in order to obtain but a few clones. 這也許需要分離出一大批雜種細胞以獲得只是小量的純系。
- Methods:Using cell clone tech nique,cell elect rophoresis and flow cytometer,we establish ed three sublines with different metast atic potential from human ovarian cancer li ne-SKOV3 (S1,S6 and S14). 方法:應用有限稀釋法對卵巢癌細胞系SKOV3進(jìn)行單細胞克隆,共分離出14個(gè)克隆亞系,根據細胞電泳率,流式細胞儀檢測所得細胞周期,篩選出3個(gè)電泳率差異較大的克隆亞系(S1、S6和S14)。
- The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer. 本研究是第一次報道在體細胞克隆和胚胎移植后成功應用嘌呤霉素標記獲得了轉強化型綠色熒光蛋白(EGFP)基因的小豬。
- Shanghai申友biotechnology company completed the study of "primer synthesis and gene sequencing," the work of the OPG (-/+) successful heterozygous mouse embryonic stem cell cloning. 上海申友生物技術(shù)公司完成了該研究中“引物合成和基因測序”的工作,成功獲得了OPG(-/+)雜合子小鼠胚胎干細胞克隆。
- RT-PCR showed that hepG2 cells were positive for B7-1 at the messenger RNA(mRNA)level. The hepG2/Hb7-1 cell clone strongly expressing B7-1(positive rate:92.2%)was picked out. 而HepG2 /hB7 1的B7 1分子陽(yáng)性表達率達到 92 6%25 ,轉染B7 1基因后較轉染前增大了 2 8倍多。
- Recent progress in handmade somatic cell cloning 徒手切割法體細胞克隆研究進(jìn)展
- The hybrid offspring of a zebra and a horse. 雜交斑馬斑馬與馬雜交的后代
- Hybridized cell lines ( CNf-A/B95-8 ) were obtained by hybridization of CNf-A cell with B95-8 cell and EBNA was detected in these hybridized cell lines. HAT培養基能殺死CNf-A細胞。 此細胞能與帶EB病毒基因的類(lèi)淋巴母細胞B95-8融合,形成的雜交細胞能在HAT培養液中存活,并可檢出EB病毒的核抗原。
- Only a fraction of 15% of the T cell clones presented obvious HBcAg-specific proliferation response. 僅15%25 (6/40)的T細胞克隆呈現明顯的HBcAg特異性淋巴細胞增殖反應。