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- Results High titer of antinuclear antibodies were found in the serum of his farther. 結果患兒父親血清中有高滴度的抗核抗體。
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 結果 得到1株穩定分泌抗體、效價(jià)高的雜交瘤細胞株,命名為SU-2000。
- The rescued VSV was characterized by indirect immune fluorescence and electron microscope, it kept the similar fast and high titer growth with parent Indiana strain. 通過(guò)免疫熒光、電鏡觀(guān)察、RT-PCR及生長(cháng)動(dòng)力學(xué)研究,證實(shí)救獲病毒保持典型的VSV印第安納株特點(diǎn)。
- We used the purified recombinant protein expressed from prokaryotic cells to immunize the New Zealand whit rabbit and achieve the high titer and superiority antisera. 以前研究發(fā)現PRRSV、IBV、MHV和TGEV等冠狀病毒的N蛋白都定位在細胞質(zhì)與核仁中,這可能是冠狀病毒的共有特征。
- Conclusion: 1.The prediction of antigen epitope is accurate. High titer antibodies of anti-GPBB were obtained with the compound peptide immuning animals. 結論:1.本文以多參數預測的抗原表位比較準確,以此為根據合成的復合肽抗原免疫動(dòng)物制備了高效價(jià)的抗體,抗體即可與天然抗原反應,也具有高度的特異性。
- Using highly pure C-reactive protein(CRP) to immunize rabbits, a high titer anti-CRP anti-serum was obtained. 采用高純度的C反應蛋白 (CRP)免疫兔 ,獲取高效價(jià)的CRP抗體。
- With the rising of HBsAg titer, positive ratio of HBeAg and HBV DNA (PCR) rises obviously. However, when in low titer, there still be positive of the last two index; when in very high titer, the positive ratio of the last two index only exceeds 70%. 結果 :隨HBsAg滴定度升高 ,HBeAg和HBV DNA(pcr)陽(yáng)性率亦明顯升高 ,但低滴定度時(shí)仍有部分被檢者后兩項呈陽(yáng)性 ,而高滴定度時(shí)后兩項的陽(yáng)性率僅略高于 70 %25。
- These findings indicated that the functions of EBV activated B lymphocytes of NPC patients and high titer of IgA/VCA individuals were lower than low titer of IgA/VCA and negative individuals in early immunological response of EBV infected PBMC. 結果表明;在EBV體外感染PBMC所引起的早期免疫反應中;NPC患體者和IgA/VCA高滴度者EBV活化的B細胞功能較IgA/VCA低滴度和陰性者降低.
- The analysis of MALDI-TOF-MS demonstrated that molecular weight is 39.4kD. The HPT protein was used to immunize rabbit for the preparation of polyclonal antibodies with high titer. MALDI-TOF-MS分析表明;HPT蛋白分子量為39.;4KD。
- Remarkable immunogenicity of the TrxA/PEB1 fusion protein was well identified: A high titer of the specific antibody to CJ Pen19 whole antigen weredemonstrated, which could cross-reacted with purified TrxA/PEB1 fusion protein. (3)證實(shí)TrxA/PEB1融合蛋白有高度特異的免疫原活性: CJ Pen19全菌抗原免疫后4周在雄性新西蘭兔誘發(fā)高滴度特異性抗體,可同時(shí)與Pen19菌體粗提蛋白和TrxA/PEB1蛋白發(fā)生特異性結合。
- High titer serum and immunoglobulin G (IgG) were prepared from specific pathogen free (SPF) chickens immunized with inactivated AIV subtype H9N2. Rabbits were immunized with this chicken anti-H9N2-AIV IgG and high titer serum were collected. 將H9N2亞型AIV增殖,經(jīng)差速離心和蔗糖密度梯度離心提純后,滅活,免疫SPF雞,制備高免血清并提純免疫球蛋白G(IgG)。 以此制備疫苗免疫家兔,制備高免血清并提純其中的IgG。
- Inthis study ,Rabies virus cell adapted strain SRV9 , were inoculated in suckling mouse brains to expand anddetected by RT-PCR . The results of TCID50 indicated that there are high titer virus in brains. 本實(shí)驗將RV SRV9 毒株接種乳鼠,發(fā)病后收集鼠腦,經(jīng)勻漿離心收取上清,RT-PCR 分析,TCID50測定證明,產(chǎn)物中含有高效價(jià)狂犬病病毒。
- The SPF chickens were incubated with oil-adjuvanted inactivated AIV vaccine, then the high titer serum was collected and the Immunoglobulin G was extracted with the saturated (NH_4)_2SO_4.And the content of the IgG is 4.090mg/mL. 將該病毒抗原制成油佐劑滅活疫苗;免疫SPF雞;收集高免血清;用(NH_4)_2SO_4法提取血清中的免疫球蛋白G(IgG);測定蛋白含量為IgG濃度為4.;090mg/mL。
- The determination of atrazine residue in water with radioimmunoassay method was studied.The High bioactive antigen was synthesized and the high titer anti-atrazine antibody was prepared. 以莠去津均三氮苯類(lèi)結構特征,合成連有活性羧基的莠去津半抗原,通過(guò)活性酯法制備出具有生物活性的人工抗原,以此免疫兔子獲得抗莠去津的多克隆抗體。
- Conclusion Dot-blot hybridization with DIG-labelled TTV DNA probe can be employed to detect TTV DNA in sera samples containing relatively high titer virus,with a sensitivity lower than that of PCR. 結論以地高辛標記的TTV探針用于斑點(diǎn)雜交可以檢出血清病毒滴度較高的TTV,其敏感度低于PCR方法。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency,high titer,good stability in target cells and low pathogenicity,and has been widely used in gene therapy. 腺病毒載體是一種非常有效的轉基因載體,具有高效率、低致病性、高滴度及不整合入宿主細胞染色體等優(yōu)點(diǎn),廣泛應用于基因治療。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer, good stability in target cells and low pathogenicity, and has been widely used in gene therapy. 摘要腺病毒載體是一種非常有效的轉基因載體,具有高效率、低致病性、高滴度及不整合入宿主細胞染色體等優(yōu)點(diǎn),廣泛應用于基因治療。
- The prepared IgY antibodies showed high titers,specificities and stabilities. 制備的抗3種咽喉菌IgY抗體滴度高,特異性強,穩定性好。
- Previously we found that DHF patients’ sera contain higher titer against TNF receptor families, including TRAIL-R1 (DR4). 先前我們的研究中發(fā)現DHF病人血清中能交互作用到腫瘤壞死因子群中的DR4。
- Less time was used for the rabbits to produce the PAbs with a higher titer and production, so the rabbit was chosen to produce PAbs in the subsequent experiments. 其中兔抗體制備的時(shí)間較短,效價(jià)高,且抗體的總量大,因此在十幾種赤潮藻的多克隆抗體制備過(guò)程中,采用家兔作為接受免疫的對象。