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- Methods We used heteroduplex mobility analysis (HMA) and sequencing to detect mixed infection. 方法使用異雙體動(dòng)態(tài)分析法和測序檢測混合感染。
- Objective To compare the effectiveness of heteroduplex, SSCP and heteroduplex-SSCP analyses in mutational screening. 目的比較異源雙鏈、單鏈構像多態(tài)(SSCP)、異源雙鏈-SSCP法篩查突變的效率，尋找簡(jiǎn)便有效的基因突變篩查方法。
- The PCR products were then analyzed by heteroduplex, SSCP and heteroduplex-SSCP analyses separately to testify the effectiveness of each method. 方法應用聚合酶鏈反應(PCR)擴增23個(gè)已知的雜合子突變，分別以異源雙鏈、SSCP、異源雙鏈-SSCP法分析突變，對比各方法對突變的檢出情況。
- Results Of the 23 mutations, 22 were detected by heteroduplex analysis (96%), 20 by SSCP analysis (87%) and 23 by heteroduplex-SSCP analysis (100%). 結果23個(gè)雜合子突變中，異源雙鏈法、SSCP法與異源雙鏈-SSCP法檢出突變分別為22個(gè)(96%25)、20個(gè)(87%25)和23個(gè)(100%25)。
- Denaturing high- performance liquid chromatography was subsequently used to screen for the presence of heteroduplexes, and DNA sequencing was applied to these heteroduplexes. 我們接著(zhù)以變性高效能液態(tài)層析法偵 測其中的異結合子，并以DNA定序的方法決定其DNA變異。
- Methods The microsatellite polymorphism of MICA exon 5 in 175 unrelated healthy individuals were investigated using PCR heteroduplex analysis. 方法用PCR-異源雙鏈分析法,對175名正常無(wú)關(guān)個(gè)體的MICA基因第5外顯子微衛星多態(tài)性的分布進(jìn)行研究。
- Conclusion Heteroduplex-SSCP analysis is simple, economical and more effective than SSCP or heteroduplex analysis alone and it may be widely applied in mutational screening. 結論異源雙鏈-SSCP法突變檢出率高，經(jīng)濟、簡(jiǎn)便，值得在基因突變篩查中推廣應用。
- Results from heteroduplex analysis are more stable and repeatable than those of SSCP and they may complement each other.Heteroduplex-SSCP analysis has the advantages of heteroduplex and SSCP analyses. 異源雙鏈法結果比SSCP法結果穩定，重復性好，兩種方法之間有一定互補性，異源雙鏈-SSCP法兼有兩種方法的特點(diǎn)。
- Methods By means of the modified heteroduplex mobility assay(HMA) and the routine DNA sequence analysis, the subtypes of 14 blood samples with HIV - 1 sera - positive were determined and analyzed and compared. 方法 采用改良的異源雙鏈泳動(dòng)法(HMA)與DNA序列分析法對14份HIV-1抗體陽(yáng)性者樣本進(jìn)行亞型測定、分析和比較。
- heteroduplex chemical mismatch cleavage 異源雙鏈化學(xué)錯配切割
- heteroduplex migration retardation 異源雙鏈的泳動(dòng)延遲
- heteroduplex mobility assay(HMA) 異源雙鏈泳動(dòng)分析技術(shù)
- unidirectional heteroduplex repair model 單向異源雙股修復模式
- Recombination: Heteroduplex and Mismatch Repair in vitro 重組:異源雙鏈和體外錯配修復
- COMPARISON OF HETERODUPLEX ANALYSIS AND SSCP IN GENE MUTATION SCREENING 異源雙鏈分析和單鏈構象多態(tài)性分析在基因突變檢測中的作用
- Rapid detection of genotypes of TT virus using a heteroduplex mobility assay 異源雙鏈泳動(dòng)分析法快速檢測TT病毒基因型
- Application of Method to Eliminate the Heteroduplex Caused in the Course of PCR to Microbial Molecular Ecology 方法在微生物分子生態(tài)學(xué)研究中的一種應用
- Application of heteroduplex mobility assay in genetic subtyping on human immunodeficiency virus type 1 異源雙鏈泳動(dòng)分析法在人類(lèi)免疫缺陷病毒1基因亞型分析中的應用
- Keywords HIV 1;genetic subtype;heteroduplex mobility assay （HMA）;molecular biology;molecular epidemiology; 人類(lèi)免疫缺陷病毒1型;基因亞型;異源雙鏈泳動(dòng)分析法;分子生物學(xué);分子流行病學(xué);
- Keywords HIV 1 Genetic subtype;heteroduplex mobility assay（HMA）;molecular epidemiolog 人類(lèi)免疫缺陷病毒1型;基因亞型;異源雙鏈泳動(dòng)分析法;分子流行病學(xué);
