The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%).
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- VH和VL重組質(zhì)粒分別轉化到大腸肝菌BL21(DE3)中,IPTG誘導后,經(jīng)SDS?PAGE電泳表明在12kD處有包含體蛋白表達,表達蛋白含量分別為28%25和35%25。