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- 本文討論了三種 DDBMS 組成方式:松散結合型(loose cou-pling)、緊密結合型(tight coupling)和集成型(integrat),并對它們的優(yōu)缺點(diǎn)進(jìn)行了分析。There are three DDBMS architectures-loose coupling,tight coupling and integrat,which will be dis- cussed in the section 2.
- 方法:采用DNA重組技術(shù)構建真核表達質(zhì)粒pTRE-Tight-CFP-CD11b和pTRE-Tight-YFP-CD18(CD11b和CD18是Mac-1的兩個(gè)亞基),通過(guò)脂質(zhì)體介導共轉染CHO細胞,用熒光顯微鏡觀(guān)察轉染后陽(yáng)性細胞;Methods: The eukaryotic expression vectors pTRE-Tight-CFP-CN11b and pTRE-Tight-YFP-CD18 were constructed by recombinant DNA technique. The 2 vectors were co-transfected into CHO cells using liposome to fuse CD11b and CD18: the 2 subunits of Mac-1. Fluorescence microscope was used to observe the cyan fluorescence and the yellow fluorescence of Mac-1-FP.
