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- SDS PAGE analysis indicated that the expressed protein was about 30 kD. SDS PAGE分析 ,表達出約 30kD大小的蛋白 ;
- The results indicated that the 97 ku expressed protein could be recognized by CSFV positive serum as expected. 結果表明,IPTG誘導表達得到的pET-NS3融合蛋白的相對分子質(zhì)量約為97 ku,與預期結果一致,且該重組蛋白能被CSFV陽(yáng)性血清所識別。
- Expressed protein of recombinant pET- 30a- P30 were correctly identified by the application of mAb against T. gondii natural P30 antigen. 抗弓形蟲(chóng)天然P30的mAb能識別重組體pET-30a -P30的表達蛋白。
- The expressed protein yield reached 385mg/L in the 10L fermentor assayed by Coomassie brilliant blue stain G-250 method. 該條件用于指導小試（5L/10L罐），蛋白表達量達到了385mg/L；
- At the same time, the study also found differently expressed protein mass spectra among different grades of astrocytomas. 此外，在不同級別的星形細胞瘤之間也存在差異表達的蛋白峰。
- The molecular weight of SSIC expressed protein was about 31kDa and virus-like particles with diameter of about 22nm were formed. 在酵母中成功地進(jìn)行了表達，表達產(chǎn)物(SSIC)的大小約為31kDa，并形成直徑約為22nm的病毒樣顆粒。
- SDS-PAGE profde showed a single protein band, and ELISA showed good antigenicity and specificity of the expressed protein. 獲得的表達蛋白純度達95%25，電泳顯示單一條蛋白帶，ELISA分析證實(shí)有較強的抗原性和較好的特異性。
- In order to express protein (RDE-4) of RNAi correctly, a pair of primers were designed in accordance with the reported RDE-4 gene sequences. 根據已報道的RDE-4基因序列;設計一對引物;從含C.
- The expressed protein was one-step purified to 97.53% using Ni-NTA affinity chromatography method under native condition, and with a yield of 18mg/L of induced culture. 表達產(chǎn)物經(jīng)Ni-NTA親和層析法一步純化;蛋白純度約達 97.;53%25;并可得到約18mg/L重組RTA蛋白。
- Objective: To observe the differentoial expressed protein spots in hypothalamus in yeast induced febrile rats and the yeast induced febrile rats treated with cinnamic aldehyde. 目的:觀(guān)察桂皮醛對酵母致熱大鼠的解熱作用及其對下丘腦蛋白質(zhì)組的影響。
- The expressed protein was purified by Glutath-one Sepharose 4B.A lot of target protein and a little of GST protein were obtained,the density of M2 was 5.6 mg/mL after defining the purification. 表達產(chǎn)物使用GST親和吸附柱進(jìn)行純化;獲得了大量的目的蛋白及微量的GST蛋白;使用分光光度儀確定純化后M2蛋白的濃度約為5.;6 mg/mL。
- Methods The NBL stage-specific gene T668 was expressed in E. coli and the expression protein was used to immunize the mice at an interval of 10 days. 方法 以旋毛蟲(chóng)新生幼蟲(chóng)期特異性基因T6 6 8在大腸桿菌中的表達蛋白為抗原免疫小鼠 ,每間隔 10d免疫 1次 ,共免疫 3次。
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表達產(chǎn)物為包涵體形式，溶解和折疊后，以GST親合層析色譜純化。
- Differentially expressed proteins were identified using Liquid Chromatography/Mass Spectrometry. 對差異表達的蛋白進(jìn)行質(zhì)譜分析。
- The result of SDS-PAGE indicated that the expression protein was about 40KD and reached to its peak at about 5 hours after inducing. SDS-PAGE電泳顯示;表達的融合蛋白的分子量為40KD左右;且在誘導表達5小時(shí)后表達量最多;大約占菌體總蛋白的22.;4%25。
- Among the identified differential expression protein,the expression of DJ-1 protein was decreased in DADS-treated group. 與未處理組比較,初步鑒定的差異蛋白質(zhì)DJ-1蛋白在處理組中表達下調,其功能與轉錄調節有關(guān)。
- The syndrome is marked by a reduction of maternally expressed proteins in a small section of chromosome 15, which is also usually paternally imprinted. 該病癥以母方表達的15號染色體的一個(gè)小片斷里的蛋白質(zhì)減少為特點(diǎn)，15號染色體父方通常是印跡的（不表達）。
- SPF chickens were immunized by each expressed proteins and the antibody titers of different groups were assayed by ELISA. 應用表達的蛋白免疫SPF雞，用ELISA檢測各組的抗體水平。
- Objective: To study the differentially expressed proteins in brain stem of seasickness susceptible rats and discuss its possible pathogenesis. 目的:研究暈船易感大鼠腦干蛋白質(zhì)的表達變化,探討暈船易感性可能發(fā)生機制。
- Objective: To study the differentially expressed proteins in brain stem of seasickness adaptive rats and discuss its possible pathogenesis. 目的:研究暈船易感大鼠腦干蛋白質(zhì)的表達變化,探討暈船易感性可能發(fā)生機制。
