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- Purified fusion protein was obtained. 得到純化的融合蛋白。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大腸埃希菌中以GST融合蛋白的形式得到表達。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF親和層析柱純化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用鎳柱純化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大腸桿菌中,表達重組MBP?hEra融合蛋白。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在體內外BVP22均能夠介導與其融合的蛋白在細胞間發(fā)生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用雙向免疫擴散法鑒定家兔多價(jià)抗血清的效價(jià)。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在體外對過(guò)度表達EGFR的Hep2細胞具有明顯的殺傷作用。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. (4)PEB1融合蛋白作為CJ的亞單位侯選基因工程疫苗不僅可行,而且有良好應用前景。
- Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.coli. 結果:成功克隆了CAT的基因片段,并在大腸桿菌中得到其融合蛋白的表達。
- A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. 利用基因工程技術(shù)在大腸桿菌表達成功人白細胞介素2(IL-2)和人血管生成素(Angiogenin)的融合蛋白(IL-2-Ang)。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela細胞中表達 ,并主要定位于細胞核。
- Western blot proved that the recombinant protein is the fusion protein of APE/Ref|1. Western印跡證明重組蛋白為APE ref 1融合蛋白 .
- Western-blot analysis with sera against CAV showed the fusion protein had good specificity. 在Western-blot免疫印跡分析中,3個(gè)融合蛋白與CAV陽(yáng)性血清均能發(fā)生特異性反應,表明它們都具有良好的反應性。
- The expression vector of PVS CP gene is constructed, a 58kDa fusion protein product is expressed in E. 構建了含PVS CP基因的融合蛋白原核表達載體,并在大腸桿菌(E.;coli
- The CHO cells that could steadily express at high level the CTLA-4/Ig fusion protein was selected for cloning. 篩選出了能高效穩定表達CTLA-4/Ig融合蛋白的CHO細胞;
- GAM molecule was taken out from human placenta cDNA library by using GST?gp130 fusion protein as a probe. 目的對以GST-gp130融合蛋白為探針,篩選人胎盤(pán)cDNA文庫而得到的一個(gè)分子(GAM,gp130associatedmolecule)cDNA序列進(jìn)行測定并做真核表達。
- These fusion proteins were used to immunize rabbits to raise antisera. 這些表達產(chǎn)物用于免疫動(dòng)物產(chǎn)生多克隆抗血清。
- The fusion protein could be detected by ELISA in COS 7 cell culture surpernatant. 重組真核表達載體CD5lnegl KIR2DL1轉染COS 7細胞后 ,ELISA法檢測細胞培養上清中有融合蛋白的表達。
- The fusion protein is significant for us to establish sensitive and specific ELIAS to detect Mhp. 該融合蛋白對于建立特異性和敏感性好的ELISSA方法具有重要意義