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- Keywords Influenza virus;DNA vaccine;double expression vector;M2 gene;flow cytometry;immunoprotection; 流感病毒;DNA疫苗;雙表達載體;M2基因;流式細胞術(shù);保護力;
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 標題： 圖1.;穿梭表達載體pRL_hEGF的構建。
- Digesting pLXSN-mB7-1 by enzyme XhoI and BamHI to gain the opened plasmid pLXSN-mB7-1 with ambly end and BamHI sticking end; Inserting IRES-mB7-2 gene fragment into it to gain the double expressing vector pLXSN-mB7-1-IRES-mB7-2; 3XhoI酶切質(zhì)粒pLXSN-mB7-1,末端鈍化,然后BamHI再酶切,形成含鈍末端和BamHI粘末端的開(kāi)環(huán)pLXSN-mB7-1質(zhì)粒,將IRES-mB7-2基因片段插入其中,得到pLXSN-mB7-1-IRES-mB7-2雙表達載體;
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功構建pPIC9K/Ang-1畢氏酵母表達載體。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的構建攜帶共擴增基因的CHO細胞表達載體。
- Subsequently, the P36 gene of Mhp strain ZCF23 was subcloned into the prokaryotic expression vector pGEX 6P-1 through EcoR I and Xho I double digestion, then the recombinant expression vector pGEX 6P-1-P36 was constructed and transformed into E. 序列確認后，用Ec口Rl和腸口I將P36基因從克隆載體中切出，并插入到GsT融和表達載體pGEx 6p一1相應位點(diǎn)，構建成重組表達載體pGEX 6p一1一P36，并轉化宿主菌BLZI。 陽(yáng)性克隆命名為BLZI(6P一l一P36)。
- Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA. 方法 將pCTLA 4/Ig表達質(zhì)粒轉染COS 7細胞 ,雙抗體夾心ELISA檢測細胞培養上清中融合蛋白表達 ;
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基礎上再構建重組表達載體pBI-ced9，將CED-9基因置于CaMV35S啟動(dòng)子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功構建表達載體pAC-HBs-Fc,為表達抗人HBsAg的IgG全抗體奠定了基礎。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 將該cDNA插入原核表達載體pET3d并在大腸桿菌BL21(DE3)中過(guò)量表達。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 構建人Na+ H+ 交換蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反義真核表達載體。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆獲得人骨形成蛋白 2基因 ,并得到此基因的真核表達載體 ,為人骨形成蛋白 2的表達打下了基礎。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息學(xué)分析構建人ID4基因啟動(dòng)子表達載體,以其作為研究ID4基因啟動(dòng)子表達調控分析的工作基礎。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接獲得250bp的目的基因序列,將目的基因克隆于pGAP9K,獲得組成型表達載體pGAP9K-gat。
- The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表達載體P13w4一cTB和pcWIJN分別轉化感受態(tài)根瘤土壤桿菌，經(jīng)過(guò)PcR篩選和酶切鑒定，獲得根瘤土壤桿菌轉化子。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 為了高效表達人表皮生長(cháng)因子，我們采用了家蠶桿狀病毒表達系統(Bombyx mori baculovirus expression vector system, BMBEVS)。
- The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 設計帶特定酶切位點(diǎn)的特異引物,分別克隆了麥冬BADH和CMO的編碼區片段,構建了植物表達載體pCU1303-BADH和pCU1303-CMO。
- Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 將上述基因克隆入綠色熒光蛋白(GFP)表達載體pIRES2-EGFP中,轉染人HeLa細胞,在熒光顯微鏡和電鏡下觀(guān)察轉染細胞的形態(tài)和結構。
- The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E. 將此ORF連接入質(zhì)粒pSE-380中，并在大腸桿菌JM109和BL21中表達。
- Conclusions 1. The expression vector pS2-DTA contains the EWS-FLI-1 binding sequence and the DTA gene sequence. 結論1、我們通過(guò)定向克隆的方法，成功地構建了含EWS-FLI-1特異結合序列和白喉毒素A鏈基因的真核表達載體。
