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- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法:采用多聚酶鏈反應(PCR),變性梯度凝膠電泳(DGGE)和DNA測序等方法對上海地區血友病甲基因突變進(jìn)行檢測。
- Denaturing gradient gel electrophoresis(DGGE) is an advanced molecular fingerprinting technique. 變性梯度凝膠電泳(DGGE)是近些年微生物分子生態(tài)學(xué)研究中的熱點(diǎn)技術(shù)之一。
- Gradient gel electrophoresis determine small, dense low-density lipoprotein in plasma of patients with coronary heart disease. 梯度膠電泳法測定冠心病患者血漿小密低密度脂蛋白。
- In Part one, the established methodology of separating LDL subfractions by Non-denaturing polyacrylamide gradient gel was improved. 第一部分對我科已建立的非變性聚丙烯酰胺凝膠梯度電泳法的改進(jìn)并進(jìn)行了重復性和穩定性研究。
- Method Evaluated by ERIC-PCR(Enterobacterial Repetitive Intergenic Consens-PCR) and PCR-TGGE(PCR-temperature gradient gel electrophoresis) of 16S rDNA V3 region. 方法利用ER IC-PCR基因組DNA指紋圖技術(shù)和16S rDNA V 3擴增-溫度梯度凝膠電泳(PCR-TGGE)指紋圖技術(shù)。
- Methods Denaturant gradient gel electrophoresis (DGGE) was used to scan all DNA fragments of 3 genes mentioned above in AD group (15 cases) and elderly control group (241 cases). 方法 應用DNA變性梯度凝膠電泳方法對15例AD和241例老年對照組的上述3個(gè)基因的DNA片段進(jìn)行掃描分析。
- Effects of different cropping system on diversity of soil bacterial community were studied by using denatured gradient gel electrophoresis (DGGE) analysis. 摘要 采用變性梯度凝膠電泳(DGGE )技術(shù),研究了不同種植模式對土壤細菌群落多樣性的影響。
- Additionally,a study was applied on analyses of bacteria classification relationship by PCR-DGGE, a method combining PCR and denture gradient gel electrophoresis. 粗提的土壤DNA經(jīng)進(jìn)一步提純后均可用于PCR反應,利用細菌16S rRNA基因 和抗菌肽Shiva-1基因的引物都得到了相應的目的產(chǎn)物。
- A method based on PCR was developed to amplify short tandem repeat(STR) in combination with denaturing gradient gel electrophoresis (DGGE) and silver dyeing. 應用聚合酶鏈反應擴增STR,經(jīng)變性凝膠電泳,再用銀染顯色方法,分析苯丙氨酸羥化酶(PAH)基因內含子3中TCTA重復序列的多態(tài)性。
- Genetic variations of 26 loci in 12 isozymes have been checked using isozyme electrophoresis on vertical gradient gel of polyacrylamide for the two populations of pearl oyster Pinctada martensicollected from Daya Bay and Beibu Gulf respectively. 用聚丙烯酰胺梯度凝膠垂直平板電泳技術(shù)測定了大亞灣和北部灣合浦珠母貝Pinctadamartensi野生種群12種同工酶26個(gè)基因座位的遺傳變異。
- Objective To evaluate the potential utility of temporal temperature gradient gel electrophoresis (TTGE) in detecting homoplasmic and heteroplasmic mtDNA mutations in patients with cancers. 目的評價(jià)時(shí)相溫度梯度凝膠電泳技術(shù)(TTGE)在腫瘤患者線(xiàn)粒體基因同質(zhì)性和異質(zhì)性突變檢測中的應用。
- Pulsed Field Gradient Gel Electrophoresis, 目前運用的脈沖電泳技術(shù)主要有:脈沖場(chǎng)梯度凝膠電泳
- temperature gradient gel elec-trophoresis TGGE
- SIMULATED ANNEALING WITH A POTENTIAL FUNCTION WITH DISCONTINUOUS GRADIENT ON TORUS 環(huán)面上具有間斷梯度的勢函數的模擬退火
- temperature gradient gel elec-trophoresis(TGGE) 溫度梯度凝膠電泳
- Temperature gradient gel eletrophoresis (TGGE) 溫度梯度凝膠電泳
- PCR-DGGE(denaturing gradient gel electrophoresis) PCR-DGGE
- denaturing gradient gel electrophoresis (DGGE) 變性梯度凝膠電泳
- temperature gradient gel electrophoresis(TGGE) TGGE
- denatured gradient gel electrophoresis(DGGE) 變性梯度凝膠電泳(DGGE)