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- The protocorms and regenerated plant were induced from the seeds and the pseudobulbs of Cremastra appendiculata(D. 以杜鵑蘭的種子和假鱗莖作外植體均誘導獲得原球莖及再生植株。
- The growth of callus induced from roots of RA regenerated plant was also resistant to AEC after subculture on AEC-free medium for three months. R_(AEC)再生植株根尖誘導的愈傷組織經(jīng)過(guò)3個(gè)月繼代培養也保持穩定的抗性。
- The isozyme analysis showed that the differences of bands pattern among first selfed progenies of new species and regenerated plant were very small in 4 isozyme sysytem (AAT, GDH, POD and ME). 同工酶分析結果顯示,新種自交一代在天冬氨酸轉氨酶(AAT)、谷氨酸脫氫酶(GDH)、蘋(píng)果酸酶(ME)、過(guò)氧化物酶(POD)4個(gè)同工酶系統上的譜型差異較小。
- Active cardon was effective for preventing the explant from browning and dying, MET 1.5 mg/L promoted the development of regenerated plant root, and the survival rate of transplants was more than 95%. 活性炭可有效防止外植體葉塊變褐死亡;多效唑1.;5 mg/L可促進(jìn)再生植株根的形成,使葉片變厚變短變綠,使試管苗移栽成活率達95%25以上。
- The regeneration plant was induced on MS medi a with BA. 菘藍毛狀根在含BA的MS培養基中成功地誘導出再生植株;
- The chromosome karyotypes and the chromosome variation of regenerated plants of perennial ryegrass(Lolium perenne L. 對多年生黑麥草核型及組織培養再生植株細胞染色體變異進(jìn)行了觀(guān)察研究。
- There are tetraploid and diploid regenerated plants in "Page tangelo + Valencia orange" and "Hongju tangerine + Rough lemon". 丹西紅橘+粗檸檬和佩奇橘柚+伏令夏橙2個(gè)組合的再生植株既有二倍體也有四倍體;
- Clustered shoots could grow from hairy roots in MS medium without growth regulators and then could form regenerated plants. 轉化毛狀根在無(wú)生長(cháng)調節物質(zhì)的MS培養基上培養可獲得叢狀芽,并發(fā)育成植株。
- The method for measurement of the length of stoma guard cell was proved to be a simple way of estimating ploidy of the regenerated plants. 通過(guò)測量氣孔保衛細胞長(cháng)度來(lái)評估再生植株的染色體倍性是一種簡(jiǎn)單可行的方法。
- Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 細胞核載體PB-adw、PBG-adw均采用農桿菌介導法將adw導入萵苣,細胞核轉化獲得了生長(cháng)良好的抗性萵苣植株,經(jīng)PCR、PCR-Southern、southern斑點(diǎn)雜交分析證實(shí),adw基因已整合到萵苣基因組中。
- Miniature mutants appeared in the generations of the regenerated plants were obtained in the scutellum cell of the sorghum pure Line 401-1 through immature embryo culture. 摘要以高粱純系401-l為材料,通過(guò)幼胚培養,由小盾片細胞產(chǎn)生的再生植株后代出現株形矮小、葉形窄短的突變體。
- The plant was growing at an angle. 植物呈一定角度生長(cháng)。
- Conclusion: Agrobacterium rhizogene can induce hairy roots from autotetraploid Isatis indigotica, and the obtained hairy roots can develop into regenerated plants. 結論:發(fā)根農桿菌可以誘導四倍體菘藍長(cháng)出毛狀根,經(jīng)誘導出的毛狀根具有再生完整植株的能力。
- The somaclonal variation of regeneration plant derived from mature embryo of perennial ryegrass (Lolium perenne L. were analyzed with RAPD makers. 摘要以多年生黑麥草成熟胚誘導獲得的再生植株為材料,利用RAPD標記方法對其體細胞變異進(jìn)行了研究。
- The results indicated that polymorphic variation of the regeneration plant was existed in molecular level. 結果表明多年生黑麥草體細胞無(wú)性系在分子水平發(fā)生了多態(tài)性變異。
- Vermiculite was the optimum medium for Rieger Begonia micropropagation in vitro, the survive rate of the root regeneration plant was 93.8%. 蛭石是麗佳秋海棠試管苗最佳的馴化移栽基質(zhì);其生根苗移栽成活率達到了93.;8%25。
- After his holiday he felt regenerated. 他休假之後覺(jué)得又有了精神。
- The party soon regenerated under her leadership. 該黨在她的領(lǐng)導下很快煥然一新。
- Utilizing this system, more than 50% hygromycin resistance selection efficiency was obtained with callus derived from young embryo (DREW, japonica ) and young panicle (D Shan B, indica ) as receptor, some regenerated plants were acquired. 依據該體系,以粳秈DREW 的幼愈傷組織和秈稻D汕B的幼穗愈傷組織為受體,經(jīng)農桿菌AGL1/pCAMBIA1301轉化后, 獲得了超過(guò)50%25 的潮霉素抗性愈傷篩選率, 并獲得再生植株。
- The invention could regenerate transgene strain from absolutely maximum acceptor cell, and has high transforming frequency, and little feature variation of regeneration plant. 本發(fā)明能從絕大多數基因型的受體細胞中再生出轉基因植株,且轉化頻率高,再生植株性狀變異小。