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- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 標題： 圖1.;穿梭表達載體pRL_hEGF的構建。
- HLF;sperm;liposome;glandmammary special expression vectors;transgenic animals. 01人乳鐵蛋白;精子;脂質(zhì)體;乳腺特異表達載體;轉基因動(dòng)物
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功構建pPIC9K/Ang-1畢氏酵母表達載體。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的構建攜帶共擴增基因的CHO細胞表達載體。
- Cloning of ACC Oxidase Gene of Peach and Construction of Its Plant Recombinant Expression Vectors?? 桃ACC氧化酶基因的克隆和植物表達載體的構建
- Meanwhile, expression vectors were constructed with sence and antisence of PCaM-1 cDNA. 同時(shí)構建PCaM-1的正、反義真核表達載體，以轉化花生研究CaM在胚胎發(fā)育中的作用。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基礎上再構建重組表達載體pBI-ced9，將CED-9基因置于CaMV35S啟動(dòng)子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功構建表達載體pAC-HBs-Fc,為表達抗人HBsAg的IgG全抗體奠定了基礎。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 將該cDNA插入原核表達載體pET3d并在大腸桿菌BL21(DE3)中過(guò)量表達。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 構建人Na+ H+ 交換蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反義真核表達載體。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆獲得人骨形成蛋白 2基因 ,并得到此基因的真核表達載體 ,為人骨形成蛋白 2的表達打下了基礎。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息學(xué)分析構建人ID4基因啟動(dòng)子表達載體,以其作為研究ID4基因啟動(dòng)子表達調控分析的工作基礎。
- Objective:To construct the recombinant HBV mammalian expression vectors by insertion of CpG motif and IL-2 gene respectively. 目的 :構建插入CpG序列和IL - 2基因的HBV重組真核表達載體。 方法 :采用PCR產(chǎn)物直接克隆方法 ;構建了編碼HBsAg基因的真核細胞表達載體pcDNA3.;1+ -HBsAg ;
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接獲得250bp的目的基因序列,將目的基因克隆于pGAP9K,獲得組成型表達載體pGAP9K-gat。
- The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表達載體P13w4一cTB和pcWIJN分別轉化感受態(tài)根瘤土壤桿菌，經(jīng)過(guò)PcR篩選和酶切鑒定，獲得根瘤土壤桿菌轉化子。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 為了高效表達人表皮生長(cháng)因子，我們采用了家蠶桿狀病毒表達系統(Bombyx mori baculovirus expression vector system, BMBEVS)。
- The expression vectors, Xm-6/c-myc was first constructed and their expression possibilities were examined in Alexander cells by immunocytochemistry. 首先構建Xm-6/c-myc真核表達載體，并將其轉染Alexander細胞，免疫組化實(shí)驗證明重組的c-mvc基因可在肝細胞中表達。
- The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 設計帶特定酶切位點(diǎn)的特異引物,分別克隆了麥冬BADH和CMO的編碼區片段,構建了植物表達載體pCU1303-BADH和pCU1303-CMO。
- Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 將上述基因克隆入綠色熒光蛋白(GFP)表達載體pIRES2-EGFP中,轉染人HeLa細胞,在熒光顯微鏡和電鏡下觀(guān)察轉染細胞的形態(tài)和結構。
- The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E. 將此ORF連接入質(zhì)粒pSE-380中，并在大腸桿菌JM109和BL21中表達。
