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- 目的 建立攜帶人類(lèi)淀粉樣前體蛋白瑞典型突變 (Swedish mutation of amyloid precursorprotein,APPSWE)基因的轉基因小鼠模型。Objective To prepare transgenic mice model of human (Swedish mutation of amyloid precursor protein, APP SWE ) gene. Methods By pronuclear microinjection, human APP SWE genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice.
- 目的 確認淀粉樣纖維蛋白基因 (amyloid fibrils,BRI)基因在 1對同源但轉移能力不同的肺腺癌細胞系 AGZY83- a和 Anip973中的序列并分析其表達。Objective To investigate the sequence of amyloid fibrils (BRI) gene and its expression in two lung adenocarcinoma cell lines AGZY83-a and Anip973 with the same tumor origin but different metastatic potential.
- 近年許多實(shí)驗證實(shí)C-型利鈉肽(c-type natriuretic peptide, CNP)及其受體廣泛存在于中樞神經(jīng)系統(central nervous system, CNS)。Recently, many scholar have found that c - type natriuretic pep-tide (CNP) and the receptor - CNP(R - CNP) in the central nerve system (CNS).
- 降鈣素基因相關(guān)肽(Calcitonin gene-related peptide,CGRP)是由降鈣素基因編碼的mRNA選擇性拼接后轉錄而成的37個(gè)氨基酸組成的單鏈多肽，是全身最主要的擴張血管的神經(jīng)活性多肽。Calcitonin gene-related peptide (CGRP) composed of 37 amino acids by alternative splicing of messenger RNA transcript of calcitoini gene.
- PAC2是垂體腺苷酸環(huán)化酶激活多肽(Pituitary adenylate cyclase activating polypeptide,PACAP)和血管活性腸肽(vasoactive intestinal peptide,VIP)的共同受體,介導多種重要生物學(xué)功能。VPAC2 is a co-receptor of pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) and mediates multiple bio-functions. In order to construct the CHO line expressing VPAC2 stably,pcDNA-VPAC2 was used to transfect CHO cells.
- 抗嗜鉻素A(Chromogranin A，CgA)、抗神經(jīng)元特異性烯醇化酶(Neuron specidic-enolase，NSE)記DNES細胞，抗生長(cháng)抑素(Somatostatin，SS)和抗血管活性腸肽(Vasoactive intestnal peptide，VIP)檢測DNES細胞的內含物。Neuroendocrine cells were identified by immunohistochemical staining of chromogranin A (CgA) and neuron specific enolase (NSE). Somatostatin (SS) and vasoactive intestinal peptide(VIP) were used to identify the inclusions of neuroendocrine cells.
