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- 結果 :Western印跡法證實(shí)APP18 81、APP18 65和APP18 58片段可與GM1分子結合 ,而APP18 51片段不與GM1分子相互作用。Immunoprecipitation Western blotting was used to identify the bindin g specialities between different gangliosides and APP 18-81 . The synthetic peptid es spanning residues 52-81 of APP blocked GM1 binding to APP 18-81 i n order to confirm the specific GM1-binding region within APP-NTFs.
- 法law
- Evans藍法測定大鼠肺毛細血管通透性、逆轉錄 聚合酶鏈式反應 (RT PCR)法測定肺NOSmRNA表達、Western印跡法測定肺組織NOS表達 ,同時(shí)進(jìn)行小腸組織學(xué)檢查以評價(jià)腸損傷程度。Pulmonary microvascular permeability was evaluated by Evans blue dye concentration. Intestinal injury was assessed by histological method. The expression of pulmonary NOS (iNOS and eNOS) and its?
- 無(wú)法unable
- Western印跡Western blotting
- 用法usage
- 乙醇固定對腫瘤細胞的蛋白質(zhì)提取及Western印跡的影響Effect of Ethanol Fixation on Protein Extraction and Western Blotting in Tumor Cells
- 說(shuō)法statement
- 輸入法input method
- Western印跡檢測細胞周期蛋白D1、A和B1表達的變化。Effects on the protein expression of cyclin A,B_1 and D_1 were assessed by Western Blot.
- 除法division
- 分析法analytical method
- Western印跡試驗標明,重組病毒CAV2-CGS能夠表達狂犬病毒糖蛋白。2000, 8 days later, the recombinant CAV-2-CGS virus was obtained, it can express rabies strain SRV9 glycoprotein improved by Western blotting assay.
- 商法commercial law
- DNA印跡法Southern blotting
- RNA印跡法Northern blotting
- 印跡法blotting
- 免疫組化檢測腎皮質(zhì)磷酸化CREB1(PCREB1)及增殖細胞核抗原(PCNA)的表達特征,Western印跡及RTPCR檢測腎皮質(zhì)CREB1磷酸化(活性)及mRNA的變化。Upro,ucr in urine and Glu, Scr, BUN in serum were determined. Immunohistochemistry and computer image-pattern analysis system were used to analyze expression of PCNA and p-CREB_1.Western blot were performed to valuate the expression of p-CREB_1 with their specific corresponding antibodies.
- DNA印跡法Southern blotting
- 用IPTG誘導重組大腸桿菌BL21(DE3)/pETLG1-3表達,SDS-PAGE分析表明在相對分子質(zhì)量60000處有特異性重組蛋白表達條帶,并經(jīng)Western印跡進(jìn)一步鑒定。It was cloned into the vector of pET-28a to construct the recombinant plasmid pETLG1-3.The recombinant E. coli BL21(DE3)/pETLG1-3 was induced by IPTG.