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- Methods: Using Western blotting hybridization assay, we detected VMLC1, VMLC2 in 13 patients with RHD and 7 cases of accidental deathes. 方法:采用West-ernbloting技術(shù)對13例RHD心衰患者及7例意外死亡者VMLC-1,VMLC-2含量進(jìn)行定量分析。
- Effect of labeling was detected by dot blot hybridization. 點(diǎn)雜交方法檢測探針標記效果。
- This CTB gene product was also verified by Western blot analysis. Western blotting結果確認了該條帶為CTB基因的產(chǎn)物。
- Western blot showed 60 kDa, which may be posttranslated product. Western blot 結果顯示,該基因的真核表達產(chǎn)物分子量約為60 kD,可能是翻譯后修飾的結果。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我們用常見(jiàn)的克隆載體pBR322作探針,用核酸點(diǎn)雜交和Southern吸印方法,檢測了來(lái)自173人的213例樣品。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重組真核質(zhì)粒PXJ 4 1/M2 -1轉染肺腺癌PAa細胞 ,應用RT PCR、Westernblot方法檢測表達。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探討斑點(diǎn)雜交法檢測結核分枝桿菌的臨床應用價(jià)值。
- The results of western blot analysis were in accordance with those of 2-DE. 對部分差異蛋白采用Western-blot方法在蛋白水平的驗證結果與2-DE結果基本相符。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點(diǎn)雜交證實(shí)我們制備的探針是敏感而可靠的。
- Western blot was used to analyze a panel of HSP90 client proteins expression. Western blot分析藥物對細胞通路信號蛋白及細胞周期相關(guān)蛋白的表達;
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑點(diǎn)雜交技術(shù)鑒定分枝桿菌菌種的靈敏度為92.;49%25;特異度為100%25。
- To develop a universal PCR-reverse blot hybridization assay, which aime to detect and identify pathogenic yeast rapidly and exactly. 摘要建立PCR結合寡核苷酸探針?lè )聪虬唿c(diǎn)雜交技術(shù),快速檢測及鑒定致病性酵母菌。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶鏈反應結合等位基因特異的寡核苷酸探針雜交(PCR/ASO)技術(shù)。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法:用地高辛素標記基因探針,斑點(diǎn)雜交法檢測未知和已知病原的肝炎患者75例,正常對照17例。
- Methods 2H4 and 5D3 were used to immunize mice. Ab3 in anti sera were tested by ELISA and Western blot. 方法用Ab2免疫Balb/c小鼠獲得抗血清 ,以ELISA檢測其Ab3,Westernblot分析其識別抗原的分子質(zhì)量。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 為了探究?jì)绕に?1(ET?1)對腎功能的影響和作用方式,采用斑點(diǎn)雜交和原位雜交方法對大鼠缺血60分鐘再灌注腎組織ET?1及其受體亞型(ETA、ETB)的基因表達進(jìn)行了研究。
- HIV test. If the initial test is positive, please repeat and perform Western Blot. 艾滋病病毒檢驗,如果最初的檢驗是呈陽(yáng)性,請進(jìn)行再次檢驗。
- Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法:用LE11.;8、MYO和M13探針,經(jīng)Southern雜交法檢測12例兒童急性粒細胞白血病患者的外周血或骨髓細胞的基因重排。
- RT-PCR and Western blot shown that there was a decrease in the expression of BRCP mRNA and protein in SWt cells. RT-PCR和Western印跡顯示,轉染細胞中BCRP基因mRNA和蛋白質(zhì)的表達下降甚至消失。