Western Blot was used to detect the ERK 1/2 levels and ERK activation product (P-ERK1/2) levels. Cell viabilities were measured with MTT assay. Cell cytopathic effect (CPE) was used to evaluate myocardial cell lesions.

 
  • 采用Westenblot分別測定ERK1/ 2 ,ERK激活后產(chǎn)物 (P ERK1/ 2 )表達變化 ,采用MTT法檢測細胞活性 ,采用細胞病變法計算細胞病變。
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