Viruses were grown in embryonated hen eggs and the viral RNA was extracted from allantoic fluid. The partial PB1 polymerase gene fragments were amplified by RT-PCR,and the purified PCR products were sequenced using the CEQ sequence kit by direct method.
英
美
- 方法 病毒在雞胚中傳代 ,自收獲的尿囊液提取RNA ,通過(guò)RT-PCR ,擴增聚合酶PB1基因片段 ,并進(jìn)行序列測定 ,測序結果采用PHYLIP軟件在Internet網(wǎng)上分析處理 ,并用TreeView軟件繪制系統進(jìn)化樹(shù)。
