Tryptophan(Trp) in cysteine proteinase inhibitor(CPI) from wheat seed was modified with N Bromosuccinimide(NBS). The data showed that there is one Trp in CPI, the modified CPI lost its inhibitory activity on papain completely.
英
美
- 用N-溴代丁烯二酰亞胺修飾小麥巰基蛋白酶抑制劑(CPI),計算出CPI分子內有一個(gè)色氨酸殘基被修飾,修飾的CPI對木瓜蛋白酶的抑制活性全部喪失,表明色氨酸對CPI的活性非常重要,是分子保持抑制活性所必需的;