Total serum bile acids were extracted and purified bySEP-PAK C18 Cartridge and were grouped with anion exchange resin PHP LH-20, conjugated bile acids were converted to free form by alkaline hydrolysisunder high pressure and temperature.

 
  • 血清膽汁酸經(jīng)SEP-PAK C_(18)小柱固相提取、凈化,用PHP LH-20柱進(jìn)行分組,結合型的膽汁酸在高溫高壓下水解為游離型。
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