To understand the mechanism of ganglioside GM 3 effecting on protein kinase C (PKC), the translocation of PKC isoforms in J6 2 cells and the content of diacylglycerol (DAG) was compared between cells treated with GM 3 and control cells.
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- 應用SDS PAGE及Western印跡技術(shù)檢測神經(jīng)節苷脂GM3 處理前后人白血病J6 2細胞不同類(lèi)型PKC在細胞內的轉位情況 ；同時(shí)利用高效薄板層析技術(shù)觀(guān)察了細胞內DAG含量的變化 ；從而探討GM3 抑制PKC活性的機制 .
