To determine which transmembrane domain could locate PF40 in the ER, 4 truncated PF40 fragments were amplified by PCR and fused with gfp.
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為了確定PF40的內質(zhì)網(wǎng)定位區段,構建了pf40的4個(gè)不同長(cháng)度的缺失片段(f12、f14、f56、f36)與報告基因gfp連接的植物表達載體,由農桿菌介導轉化煙草。
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