To construct the recombinant SFV containing the PrM E gene, the SFV expression vector DNA, which contained the PrM E gene of Chinese dengue 2 virus, and the helper vector DNA were linearized and transcribed in vitro , respectively.

 
  • 為構建含PrM E基因的重組SFV病毒 ;將含我國登革 2型病毒PrM E基因的SFV表達載體和輔助載體DNA線(xiàn)性化后 ;進(jìn)行體外轉錄 .
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