Tie template RNA of Cucumber mosaic virus 2-1-2 isolated from Echinacea purpurea Moench was amplified by RT-PCR,the amplified fragments were cloned and sequenced.

　
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  • 以紫松果菊上分離的黃瓜花葉病毒2-1-2的RNA為模板,采用RT-PCR(反轉錄聚合酶鏈式反應,下同)技術(shù)對2-1-2的外殼蛋白(coat prote in,CP)基因進(jìn)行了克隆和序列測定。
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