There was only one mutant , the NO. 880 base to down-side the translation stop codon (TAA).But the mutant was dispeared when conpare it with the sequence of Streptococcus mutans reported in Genebank.
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- 本實(shí)驗將反向PCR方法與核酸內切酶設計相結合誘導變形鏈球菌乳酸脫氫酶基因缺失突變，實(shí)驗證實(shí)該方法只需設計一對反向PCR引物，進(jìn)行一輪PCR反應，一次酶切連接反應，方法簡(jiǎn)便快捷。
