The smaller truncated P32 fragment without the transmembrane region of P32 was amplified by PCR, and the E. coli expression vector pPRP-TRUNCP32 was constructed. A fusion protein nearly 31kDa was expressed by IPTG-induced BL21(DE3).
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- 以PCR方法擴增了切去跨膜結構的P32基因片段,構建了表達部分P32基因的大腸桿菌表達載體pPRO-TRUNCP32,轉化大腸桿菌BL21(DE3),經(jīng)誘導表達出特異的31kDa融合蛋白,蛋白以包涵體形式存在。