The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation.

 
  • 將兩種候選蛋白的編碼序列分別克隆于上述融合載體,轉染Cos?7細胞后的表達產(chǎn)物,可利用抗HA或myc的mAb進(jìn)行Western?blot和免疫共沉淀。
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