The ribosomal internal transcribed spacer regions (ITS 1 and ITS 2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T vectors, cloned and sequenced.
英
美
- 以相應引物經(jīng)PCR擴增了太平洋牡蠣 (Crassostreagigas)的核糖體轉錄間區域 (ITS 1和ITS 2 )及線(xiàn)粒體 16SrDNA和COI基因片段。 PCR產(chǎn)物經(jīng)T 載體連接后進(jìn)行克隆和測序 ,分別得到長(cháng)度為 5 4 3、791、5 30和 70 0bp的核苷酸序列。
