The promoter that RNA polymerase III recognize are U6 promoter or H1 promoter, therefore the key step of plasmid-based RNA interference technology is cloning the RNA interference target sequence to downstream of U6 or H1 promoters.
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- RNA聚合酶識別的是U6或H1啟動(dòng)子,所以將RNA干擾序列構建到這些啟動(dòng)子的下游是以質(zhì)粒載體為基礎的RNA干擾技術(shù)的關(guān)鍵步驟。