The positive clones were screened by G418.The proliferation,migration,and invasion of MDA-MB-231 cells before and after transfection were assessed by CFSE-flow cytometry,monolayer wound healing assay,and soft agar colony formation test.

 
  • 應用CFSE-流式細胞儀檢測,單層傷口愈合實(shí)驗,軟瓊脂克隆形成實(shí)驗等方法觀(guān)察了MDA-MB-231細胞轉染前后,細胞體外增殖及侵襲能力等指標的變化。
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