The karyotes were separated by density gradient centrifugation, and the stroma cells of the bone marrow were gained adherent separation method.

 
  • 選擇雄性6周齡SD大鼠4只,抽取大鼠股骨骨髓,使用密度梯度離心分離有核細胞,貼壁分離法獲取骨髓基質(zhì)細胞,以2×104/cm2的密度接種進(jìn)行細胞傳代與分組實(shí)驗。
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