The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively.
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- 采用逆轉錄－多聚酶鏈反應（RT－PCR）的方法從麻疹病毒Edmonston株基因組中擴增出血凝素H基因和融合蛋白F基因，并利用轉移質(zhì)粒將這兩個(gè)基因分別重組到桿狀病毒多角體蛋白啟動(dòng)子（PH）控制之下，獲得重組病毒vBMVH和vBMVF。
