The full-length cDNA clone, NDV3GM122, and the three helperplasmids pCI-NP, pCI-P and pCI-L of Newcastle disease virus strain ZJI isolated from an outbreak in the goose were cotransfected into BSR-T7/5 cell expressing T7 RNA polymerase.

 
  • 利用反向遺傳操作技術(shù),將ZJI株鵝源新城疫病毒全基因組cNDA克隆(NDV3GM122)和含該毒株NP、P及L基因的3個(gè)表達載體(pCI-NP、pCI-P與pCI-L)共轉染BSR-T7/5細胞;
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