The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition.

 
  • 采用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝干細胞,通過(guò)免疫細胞化學(xué)方法對其進(jìn)行初步鑒定,以及應用流式細胞儀等對其生長(cháng)狀況進(jìn)行評估。
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