The decreased activity by 50 fold and the kinetic isotope effects show that CH1 bond cleavage is more rate-limited in the Y429F mutant, consistent with the proposed proton shuttling by Tyr429.

 
  • 其中Y429F突變株酵素活性下降約50倍,且從其動(dòng)力學(xué)同位素效應結果顯示,Y429的突變使CH1鍵結斷裂的速率更受到限制,乃符合Y429作為活性中心堿基之推測。
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