The best expansion conditions for MSC in vitro was 1 l04/ml cell density, 10%FBS, 10ng/ml EGF and 10ng/ml PDGF-BB. After the induction of RA, MSC differentiated into neurons without dopaminergic neuron phenotype.

 
  • 以接種密度為1×10~4個(gè)/ml、加入10%25胎牛血清、10ng/ml EGF和10ng/ml PDGF-BB的培養條件最有利于MSC的增殖;
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