The active anti-AIF scFv was recovered efficiently from inclusion bodies with a refolding yield of approximate 75% confirmed by spectrophotometer.

 
  • AIF4c9scFv表達蛋白在細胞質(zhì)中主要以不溶性包涵體形式存在,優(yōu)化后的柱內復性程序可從高濃度的包涵體變性蛋白中高效復性AIF4c9scFv表達蛋白,其回收率可達75%25左右。
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