The IEF PAGE showed that the wild type and M222A mutant had the same pI of 8.92,while those of double mutant,triple mutant and tetra mutant were 8.88,9.10 and 9.17 respectively.

 
  • 野生型堿性蛋白酶與(M222A)突變種的等電點(diǎn)均為8?92,而(M222A,N118S),(M222A,N118S,Q103R)和(M222A,N118S,Q103R,D60N)突變酶分別為8?88,9?10和9?17。
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